Batiuskaite Danute, Grinceviciute Nora, Snitka Valentinas
Research Center for Microsystems and Nanotechnology, Kaunas University of Technology, 65 Studentu str., Kaunas LT-51369, Lithuania; Department of Biology, Faculty of Natural Sciences, Vytautas Magnus University, 58 K. Donelaicio str., Kaunas LT-44248, Lithuania.
Research Center for Microsystems and Nanotechnology, Kaunas University of Technology, 65 Studentu str., Kaunas LT-51369, Lithuania.
Toxicol In Vitro. 2015 Aug;29(5):1195-200. doi: 10.1016/j.tiv.2015.05.004. Epub 2015 May 15.
The evaluation of the cyto- and bio-compatibility is a critical step in the development of graphene oxide (GO) as a new promising material for in vivo biomedical applications. In this study, we report the impact of GO, with and without the addition of bovine serum albumin, on healthy (Chinese hamster ovary) and a cancer (mouse hepatoma MH-22A) cells viability and the estimation of the intracellular distribution of GO inside the cells in vitro. The viability tests were performed using a colony formation assay. The intracellular distribution of GO was estimated using Raman spectroscopy and imaging. The viability of both cell lines decreased with increasing concentration of graphene oxide (12.5-50.0 μg/ml): in the case of Chinese hamster ovary cells viability decreased from 44% to 11%, in the case of mouse hepatoma MH-22A cells--from 22% to 3%. These cell lines significantly differed in their response to GO and GO-BSA formulations. The results of viability tests correlate with results of atomic force microscopy and Raman spectroscopy and imaging findings. The GO influence on cell morphology changes, cell structure, cells colony growth dynamics and GO accumulation inside the cells was higher in the case of mouse hepatoma MH-22A cells.
评估细胞相容性和生物相容性是氧化石墨烯（GO）作为一种用于体内生物医学应用的新型有前景材料开发过程中的关键步骤。在本研究中，我们报告了添加和未添加牛血清白蛋白的GO对健康（中国仓鼠卵巢）细胞和癌症（小鼠肝癌MH - 22A）细胞活力的影响，以及体外细胞内GO的分布估计。使用集落形成试验进行活力测试。使用拉曼光谱和成像估计GO在细胞内的分布。两种细胞系的活力均随着氧化石墨烯浓度（12.5 - 50.0μg/ml）的增加而降低：对于中国仓鼠卵巢细胞，活力从44%降至11%；对于小鼠肝癌MH - 22A细胞，活力从22%降至3%。这些细胞系对GO和GO - BSA制剂的反应存在显著差异。活力测试结果与原子力显微镜结果以及拉曼光谱和成像结果相关。在小鼠肝癌MH - 22A细胞中，GO对细胞形态变化、细胞结构、细胞集落生长动力学和细胞内GO积累的影响更大。
