Morales M, Flores C, Pino K, Angulo J, López-Lastra M, Castro-Rodriguez J A
Department of Pediatrics, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.
Molecular Virology Laboratory/Instituto Milenio de Inmunologia e Inmunoterapia, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.
Allergol Immunopathol (Madr). 2016 Jan-Feb;44(1):59-65. doi: 10.1016/j.aller.2015.02.003. Epub 2015 May 13.
Urinary leukotriene (LTE4) is an important marker of airway inflammation presence. A relationship between single nucleotide polymorphism in the glucocorticoid receptor (GCR) gene promoter (Bcl I polymorphism), development of asthma and sensitivity to glucocorticoids has been hypothesised.
To explore the possible association between the Bcl I polymorphism and baseline levels of urinary LTE4 in preschoolers with recurrent wheezing episodes. We prospectively enrolled and classified 86 preschoolers based on the risk of developing asthma (by the Asthma Predictive Index [API]).
At admission standardised questionnaires for demographics and respiratory illness characteristics were completed. The Bcl I polymorphism of the GCR was determined by a PCR-RFLP assay from blood samples, and urinary leukotriene was assessed from urine samples by an enzyme immunoassay.
We enrolled 86 preschoolers (46 with positive API and 40 with negative API). There were no statistical differences in demographic, respiratory illnesses and wheezing episodes characteristics between both groups. Also, the prevalence of Bcl I polymorphism was similar between positive vs. negative API groups (34.8% vs. 38.9% for homozygote GG, 56.5% vs. 52.8% for heterozygote GC, 8.7% vs. 8.3% for homozygote CC, respectively, p=0.94). However, urinary LTE4 (median [IQR]) was higher in preschoolers with positive than negative API (7.18 [5.57-8.96pg/ml] vs. 6.42 [3.96-8.07pg/ml], p=0.02, respectively).
In our population, wheezing preschoolers with positive API exhibit higher levels of urinary LTE4 than those with negative API; but there were no differences in Bcl I polymorphism of the GCR.
尿白三烯（LTE4）是气道炎症存在的重要标志物。糖皮质激素受体（GCR）基因）基因启动子中的单核苷酸多态性（Bcl I多态性）、哮喘的发生与对糖皮质激素的敏感性之间的关系已被提出。
探讨Bcl I多态性与反复喘息发作的学龄前儿童尿LTE4基线水平之间的可能关联。我们根据哮喘发生风险（通过哮喘预测指数[API]）对86名学龄前儿童进行了前瞻性招募和分类。
入院时完成了关于人口统计学和呼吸道疾病特征的标准化问卷。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析从血样中测定GCR的Bcl I多态性，并通过酶免疫测定法从尿样中评估尿白三烯。
我们招募了86名学龄前儿童（46名API阳性和40名API阴性）。两组在人口统计学、呼吸道疾病和喘息发作特征方面无统计学差异。此外，Bcl I多态性的患病率在API阳性组与阴性组之间相似（纯合子GG分别为34.8%对38.9%，杂合子GC分别为56.5%对52.8%，纯合子CC分别为8.7%对8.3%，p = 0.94）。然而，API阳性的学龄前儿童尿LTE4（中位数[四分位间距]）高于API阴性的儿童（分别为7.18 [5.57 - 8.96pg/ml]对6.42 [3.96 - 8.07pg/ml]，p = 0.02）。
在我们的研究人群中，API阳性的喘息学龄前儿童尿LTE4水平高于API阴性的儿童；但GCR的Bcl I多态性无差异。
