Jia Hongmin, Gao Xue, Shi Yu, Sayyadi Nima, Zhang Zhiqiang, Zhao Qi, Meng Qingtao, Zhang Run
School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114044, China.
Department of Chemistry and Biomolecular Sciences, Faculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.
Spectrochim Acta A Mol Biomol Spectrosc. 2015;149:674-81. doi: 10.1016/j.saa.2015.04.111. Epub 2015 May 8.
Although ferric ion (Fe(3+)) performs critical roles in diverse biochemical processes in living systems, its physiological and pathophysiological functions have not been fully explored due to the lack of methods for quantification of Fe(3+) ions in biological system. In this work, a highly sensitive and selective fluorescence chemosensor, L, was developed for the detection of Fe(3+) ions in aqueous solution and in living cells. L was facile synthesized by one step reaction and well characterized by NMR, API-ES, FT-IR, and elementary analysis. The prepared chemosensor displayed excellent selectivity for Fe(3+) ions detection over a wide range of tested metal ions. In the present of Fe(3+) ions, the strong green fluorescence of L was substantially quenched. The 1:1 stoichiometry of the complexation was confirmed by a Job's plot. The association constant (Ka) of L with Fe(3+) was evaluated using the Benesi-Hildebrand method and was found to be 1.36×10(4) M(-1). The MTT assay determined that L exhibits low cytotoxicity toward living cells. Confocal imaging and flow cytometry studies showed that L is readily interiorized by MDA-MB-231 cells through an energy-dependent pathway and could be used to detect of Fe(3+) ions in living cells.
尽管铁离子（Fe(3+)）在生命系统的多种生化过程中发挥着关键作用，但由于缺乏生物系统中Fe(3+)离子的定量方法，其生理和病理生理功能尚未得到充分探索。在这项工作中，开发了一种高灵敏度和选择性的荧光化学传感器L，用于检测水溶液和活细胞中的Fe(3+)离子。L通过一步反应简便合成，并通过核磁共振（NMR）、大气压化学电离质谱（API-ES）、傅里叶变换红外光谱（FT-IR）和元素分析进行了充分表征。制备的化学传感器在广泛的测试金属离子中对Fe(3+)离子检测表现出优异的选择性。在Fe(3+)离子存在下，L的强烈绿色荧光被显著猝灭。通过Job曲线证实了络合的1:1化学计量比。使用Benesi-Hildebrand方法评估了L与Fe(3+)的缔合常数（Ka），发现其为1.36×10(4) M(-1)。MTT法测定L对活细胞表现出低细胞毒性。共聚焦成像和流式细胞术研究表明，L通过能量依赖途径很容易被MDA-MB-231细胞内化，并可用于检测活细胞中的Fe(3+)离子。
