MICA和NKG2D在人肾癌细胞中的调控作用。
Regulation Roles of MICA and NKG2D in Human Renal Cancer Cells.
作者信息
Jia Hong-Ying, Liu Jun-Li, Yuan Ming-Zhen, Zhou Cheng-Jun, Sun Wen-Dong, Zhao Jing-Jie, Wang Jue, Liu Ling, Luan Yun
机构信息
Clinical Molecular Biology Laboratory, the Second Hospital of Shandong University, Jinan, Shandong Province, China E-mail :
出版信息
Asian Pac J Cancer Prev. 2015;16(9):3901-5. doi: 10.7314/apjcp.2015.16.9.3901.
OBJECTIVE
Our aim was to investigation the roles of MHC class I chain-related gene A(MICA) and natural killer cell group 2D(NKG2D) in human renal cancer cells.
MATERIALS AND METHODS
The expression of membrane MICA (mMICA) on renal cells and NKG2D on NK cells were detected by flow cytometry (FCM); the content of sMICA were detected by enzyme linked immunosorbent assay (ELISA) and the distribution of mMICA on renal tumor tissues by immunohistochemistry; the interaction between MICA and NKG2D was observed by antibody closed method.
RESULTS
Our results showed that the expression of mMICA in renal cancer tissues was significantly higher than in controls, where the soluble MICA was not expressed. Cytotoxic activity of NK cells was significantly reduced after exposure to NKG2D and MICA antibodies (P<0.05), and serum containing sMICA can obviously lower the function of NKG2D (P<0.05).
CONCLUSIONS
The interaction of mMICA and NKG2D play important roles in mediation of cytotoxicity of NK cells in RCC. On the other hand, sMICA may mediate tumor immune escape through down- regulated NKG2D expression.
目的
我们的目的是研究MHC I类链相关基因A（MICA）和自然杀伤细胞2D组（NKG2D）在人肾癌细胞中的作用。
材料与方法
采用流式细胞术（FCM）检测肾细胞上膜型MICA（mMICA）和自然杀伤细胞上NKG2D的表达；采用酶联免疫吸附测定（ELISA）检测可溶性MICA（sMICA）的含量，采用免疫组织化学法检测mMICA在肾肿瘤组织中的分布；采用抗体封闭法观察MICA与NKG2D之间的相互作用。
结果
我们的结果显示，肾癌组织中mMICA的表达明显高于对照组，而对照组中未表达可溶性MICA。自然杀伤细胞暴露于NKG2D和MICA抗体后，其细胞毒活性显著降低（P<0.05），含sMICA的血清可明显降低NKG2D的功能（P<0.05）。
结论
mMICA与NKG2D的相互作用在肾细胞癌中自然杀伤细胞细胞毒性的介导中起重要作用。另一方面，sMICA可能通过下调NKG2D表达介导肿瘤免疫逃逸。
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