地塞米松和钙对肾上腺切除大鼠降钙素mRNA水平的相加作用。

Additive effects of dexamethasone and calcium on the calcitonin mRNA level in adrenalectomized rats.

作者信息

Besnard P, Jousset V, Garel J M

机构信息

Endocrinologie Moléculaire et Métabolique, Université Pierre et Marie Curie, Paris, France.

出版信息

FEBS Lett. 1989 Dec 4;258(2):293-6. doi: 10.1016/0014-5793(89)81677-1.

DOI:10.1016/0014-5793(89)81677-1

PMID:2599094

Abstract

Northern hybridizations were used to evaluate the effects of dexamathasone and calcium on calcitonin mRNA levels in adrenalectomized female rats. Two weeks after adrenalectomy, a 3.6-fold decrease in the calcitonin mRNA level was observed (28% vs 100% in sham-operated controls). After 5 days of dexamethasone treatment (1.5 mg/kg b. wt), a 2.6-fold rise in calcitonin mRNA occurred in adrenalectomized rats (73% vs 28%). This increment was higher when dexamethasone treated animals were injected with calcium (100% vs 73%). The effect of calcium on the calcitonin mRNA level of adrenalectomized rats treated or not with dexamethasone was similar, and additive in the former case. Our data suggest that calcium and dexamethasone elevate calcitonin mRNA by two different mechanisms.

摘要

采用Northern杂交技术评估地塞米松和钙对肾上腺切除的雌性大鼠降钙素mRNA水平的影响。肾上腺切除术后两周，观察到降钙素mRNA水平下降了3.6倍（假手术对照组为100%，而肾上腺切除组为28%）。地塞米松治疗5天（1.5mg/kg体重）后，肾上腺切除大鼠的降钙素mRNA水平升高了2.6倍（从28%升至73%）。当给地塞米松治疗的动物注射钙时，这种升高更为明显（从73%升至100%）。钙对肾上腺切除且接受或未接受地塞米松治疗的大鼠降钙素mRNA水平的影响相似，且在前一种情况下具有叠加作用。我们的数据表明，钙和地塞米松通过两种不同机制提高降钙素mRNA水平。