Chernajovsky Y
Department of Immunology, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
FEBS Lett. 1989 Dec 4;258(2):323-30. doi: 10.1016/0014-5793(89)81685-0.
Proteins in nuclear extracts of HeLa cells that constitutively bound in vitro to three regions upstream of the interferon-inducible gene 6-16 were separated partially by chromatography on DEAE-Sepharose. Region one, a CCAAT box in the non-coding strand at position --63 to --67, was protected from DNase digestion by the bound protein(s) and was required for transcription in vitro. Region two, a tandem duplication sequence at position --89 to --168 contains two copies of a sequence essential for strong induction of the 6-16 gene by interferon in vitro. Region three, a palindromic sequence at position --449 to --465, not necessary for induction of 6-16 by interferon, was also protected from DNase digestion by nuclear protein(s). Templates with or without regions of two and three were transcribed equally well in extracts from interferon-treated or untreated cells.
通过DEAE-琼脂糖层析对HeLa细胞核提取物中与干扰素诱导基因6-16上游三个区域组成性体外结合的蛋白质进行了部分分离。区域一，位于-63至-67位置非编码链上的一个CCAAT框，被结合蛋白保护免受DNase消化，并且是体外转录所必需的。区域二，位于-89至-168位置的串联重复序列包含两个对干扰素体外强烈诱导6-16基因至关重要的序列拷贝。区域三，位于-449至-465位置的回文序列，对干扰素诱导6-16不是必需的，也被核蛋白保护免受DNase消化。含有或不含有区域二和区域三的模板在干扰素处理或未处理细胞的提取物中转录效果相同。
