Ishikawa T, Kobayashi K, Sogame Y, Hayashi K
Department of Biochemistry, Osaka University Medical School, Japan.
FEBS Lett. 1989 Dec 18;259(1):95-8. doi: 10.1016/0014-5793(89)81503-0.
Using rat heart sarcolemma and liver plasma membrane vesicles, it has been verified that the transport of leukotriene C4 (LTC4) across membranes is an ATP-dependent process; the apparent Km for LTC4 was 150 nM (heart sarcolemma) or 250 nM (liver plasma membrane). S-(2,4-dinitrophenyl)-glutathione (DNP-SG) inhibited LTC4 uptake into the vesicles dose-dependently (I50 = 25 microM for both heart sarcolemma and liver plasma membrane vesicles). Mutual inhibition between LTC4 and DNP-SG in uptake into the vesicles demonstrates that transport of LTC4 is mediated by an ATP-dependent glutathione S-conjugate carrier.
利用大鼠心脏肌膜和肝细胞膜囊泡，已证实白三烯C4（LTC4）跨膜转运是一个依赖ATP的过程；LTC4的表观Km值为150 nM（心脏肌膜）或250 nM（肝细胞膜）。S-（2,4-二硝基苯基）-谷胱甘肽（DNP-SG）剂量依赖性地抑制LTC4摄入囊泡（心脏肌膜和肝细胞膜囊泡的I50均为25 μM）。LTC4与DNP-SG在摄入囊泡过程中的相互抑制表明，LTC4的转运是由一个依赖ATP的谷胱甘肽S-结合物载体介导的。
