转化生长因子-β对体外视网膜色素上皮细胞的影响。
Effects of TGF-beta on retinal pigmented epithelium in vitro.
作者信息
Opas M, Dziak E
机构信息
Department of Anatomy, University of Toronto, Canada.
出版信息
Exp Cell Biol. 1989;57(4):206-12. doi: 10.1159/000163527.
Embryonic chick retinal pigmented epithelium (RPE) has been grown on glass derivatized with covalently bound proteins of basement membrane and treated with transforming growth factor-beta (TGF-beta). In the present paper we show that over the concentration range tested (0.1-10 ng/ml) TGF-beta has no effect on RPE cell proliferation either in the presence or the absence of serum, cell motility and the organization of cytoskeleton-extracellular matrix linkage complexes with respect to their structure and presence of actin, vinculin, talin, integrin and fibronectin. The protein profiles of total cell/ECM extracts of cells grown in the presence or the absence of TGF-beta are similar although some stimulation of protein synthesis and of production of fibronectin-containing extracellular matrix has been detected.
胚胎鸡视网膜色素上皮细胞（RPE）已在经共价结合基底膜蛋白衍生化并经转化生长因子-β（TGF-β）处理的玻璃上生长。在本文中，我们表明在所测试的浓度范围（0.1 - 10 ng/ml）内，无论有无血清，TGF-β对RPE细胞增殖、细胞运动性以及细胞骨架 - 细胞外基质连接复合物的组织（就其结构以及肌动蛋白、纽蛋白、踝蛋白、整合素和纤连蛋白的存在而言）均无影响。尽管已检测到在有或无TGF-β存在的情况下生长的细胞的总细胞/细胞外基质提取物的蛋白质谱相似，但蛋白质合成和含纤连蛋白的细胞外基质产生有一些刺激作用。
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