Bentes Gentil Arthur, Lanzarini Natália Maria, Lima Lyana Rodrigues Pinto, Manso Pedro Paulo de Abreu, da Silva Alexandre Dos Santos, Mouta Junior Sergio da Silva E, Guimarães Juliana Rodrigues, de Moraes Marcia Terezinha Baroni, Pelajo-Machado Marcelo, Pinto Marcelo Alves
Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
Laboratório de Patologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz. 2015 Jun;110(4):577-9. doi: 10.1590/0074-02760140457. Epub 2015 May 15.
An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.
由于使用免疫球蛋白Y（IgY）在诊断测试方面具有诸多优势，包括易于获取、成本低廉、可转化为大规模生产，且其生产符合伦理道德，因此针对IgY的研究越来越多。在之前的一项工作中，从对甲型肝炎病毒（HAV）抗原具有反应性的蛋黄中制备并纯化了免疫球蛋白（IgY）。在本研究中，这种抗HAV特异性IgY被用于间接免疫荧光试验，以检测从实验感染的食蟹猴获得的肝活检组织中的病毒抗原。使用共聚焦显微镜在肝脏切片中检测到了HAV抗原呈阳性的区域。总之，免疫母鸡所产的蛋黄可能是生产抗体的可靠来源，可用于免疫学研究。
