通过DNA-DNA杂交评估的肋生弧菌菌株之间的关系。

Relationships among Vibrio costicola strains assessed by DNA-DNA hybridization.

作者信息

Gutiérrez M C, García M T, Ventosa A, Ruiz-Berraquero F

机构信息

Department of Microbiology, Faculty of Pharmacy, University of Sevilla, Spain.

出版信息

FEMS Microbiol Lett. 1989 Oct 1;52(1-2):37-40. doi: 10.1016/0378-1097(89)90166-3.

DOI:10.1016/0378-1097(89)90166-3

PMID:2599359

Abstract

Deoxyribonucleic acid (DNA)-DNA hybridization was used to determine the relationships among 14 strains of Vibrio costicola isolated from cured meats or hypersaline waters. Our results indicate that the strains investigated should be considered members of the same genomic species, with homologies of 60% or higher with the V. costicola strains used as reference. Low DNA relatedness was found with other marine vibrios or moderately halophilic bacteria tested. The guanine-plus-cytosine base contents of DNAs from the 14 V. costicola strains were 47.6 to 51.1 mol%.

摘要

采用脱氧核糖核酸（DNA）-DNA杂交技术来确定从腌制肉类或高盐度水域分离出的14株肋状弧菌之间的关系。我们的结果表明，所研究的菌株应被视为同一基因组种的成员，与用作参考的肋状弧菌菌株的同源性为60%或更高。与测试的其他海洋弧菌或中度嗜盐细菌相比，发现其DNA相关性较低。14株肋状弧菌菌株的DNA中鸟嘌呤加胞嘧啶碱基含量为47.6%至51.1%（摩尔）。