酿酒酵母利用内源性木糖同化基因进行木糖发酵。
Xylose fermentation by Saccharomyces cerevisiae using endogenous xylose-assimilating genes.
作者信息
Konishi Jin, Fukuda Akira, Mutaguchi Kozue, Uemura Takeshi
机构信息
Bio Fuels & Chemicals R&D Group, Frontier Research Laboratory, Central Technical Research Laboratory, JX Nippon Oil & Energy Corporation, 8, Chidori-cho, Naka-Ku, Yokohama, 231-0815, Japan,
出版信息
Biotechnol Lett. 2015 Aug;37(8):1623-30. doi: 10.1007/s10529-015-1840-2. Epub 2015 May 21.
OBJECTIVES
To genetically engineer Saccharomyces cerevisiae for improved ethanol productivity from glucose/xylose mixtures.
RESULTS
An endogenous gene cassette composed of aldose reductase (GRE3), sorbitol dehydrogenase (SOR1) and xylulose kinase (XKS1) with a PGK1 promoter and a terminator was introduced into two S. cerevisiae strains, a laboratory strain (CEN.PK2-1C) and an industrial strain (Kyokai No. 7). The engineered Kyokai No. 7 strain (K7-XYL) exhibited a higher sugar consumption rate (1.03 g l(-1) h(-1)) and ethanol yield (63.8 %) from a glucose and xylose mixture compared to the engineered CEN.PK2-1C strain. Furthermore, K7-XYL produced a larger amount of ethanol (39.6 g l(-1)) compared to K7-SsXYL (32 g l(-1)) with integrated xylose reductase and xylitol dehydrogenase from a xylose-assimilating yeast Scheffersomyces stipitis instead of GRE3 and SOR1.
CONCLUSION
The created S. cerevisiae strain showed sufficient xylose-fermenting ability to be used for efficient ethanol production from glucose/xylose.
目的
对酿酒酵母进行基因工程改造，以提高其利用葡萄糖/木糖混合物生产乙醇的能力。
结果
将由醛糖还原酶（GRE3）、山梨醇脱氢酶（SOR1）和木酮糖激酶（XKS1）组成的内源性基因盒，连同PGK1启动子和终止子，导入两种酿酒酵母菌株，一种是实验室菌株（CEN.PK2-1C），另一种是工业菌株（7号酵母）。与经过基因工程改造的CEN.PK2-1C菌株相比，经过基因工程改造的7号酵母菌株（K7-XYL）从葡萄糖和木糖混合物中的糖消耗率更高（1.03 g l(-1) h(-1)），乙醇产率更高（63.8%）。此外，与整合了来自木糖同化酵母树干毕赤酵母的木糖还原酶和木糖醇脱氢酶而非GRE3和SOR1的K7-SsXYL（32 g l(-1)）相比，K7-XYL产生的乙醇量更多（39.6 g l(-1)）。
结论
构建的酿酒酵母菌株显示出足够的木糖发酵能力，可用于从葡萄糖/木糖高效生产乙醇。
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