Behboodi E, Katz D F, Overstreet J W, Hendrickx A G
Department of Obstetrics and Gynecology, California Primate Research Center, University of California, Davis 95616.
Gamete Res. 1989 Nov;24(3):333-42. doi: 10.1002/mrd.1120240309.
Postcoital (pc) cervical mucus was collected in 73 menstrual cycles of cynomolgus monkeys and in 43 cycles of rhesus monkeys at 2, 6, 10, 30 hr pc. Videomicrography was used to analyze sperm numbers and movement in the mucus. Both cynomolgus and rhesus monkeys had comparable populations of motile sperm in the mucus at 2 hr pc. However, by 6 hr pc, cervical mucus from cynomolgus monkeys contained twice as many total sperm and motile sperm as mucus from rhesus monkeys (P less than .05). Mean swimming speeds of the free-swimming cervical sperm were similar for the two species at this time. No motile sperm were recovered in mucus from rhesus monkeys at 30 hr pc. In cynomolgus monkeys, however, 14 of the 26 animals examined at 30 hr pc had motile sperm in their mucus. These sperm exhibited lower percent motility, percent free-swimming sperm, and swimming speed than those sperm observed at 6 hr pc. Uterine sperm were collected by transcervical or transuterine aspiration from cynomolgus monkeys. In the transcervical technique, sperm were successfully obtained in four of nine animals examined at 6 hr and in four of five animals at 30 hr pc. The percentage of motile sperm in the uterine fluid was high, 82% +/- 4%, and the swimming speeds (86 +/- 2 microns/sec) were higher than those observed in cervical mucus. Approximately 5-10% of the uterine sperm exhibited swimming motions similar to the hyperactivated motility seen in most mammals. These findings indicate that the sperm cervical mucus interaction in vivo in cynomolgus monkeys has more similarities to the human situation than does the interaction in rhesus monkeys.
在食蟹猴的73个月经周期以及恒河猴的43个月经周期中，于性交后2、6、10、30小时采集宫颈黏液。采用视频显微镜分析黏液中的精子数量和运动情况。食蟹猴和恒河猴在性交后2小时黏液中的活动精子数量相当。然而，到性交后6小时，食蟹猴宫颈黏液中的精子总数和活动精子数量是恒河猴黏液中的两倍（P<0.05）。此时，两种猴游离于宫颈的精子平均游动速度相似。在性交后30小时，恒河猴的黏液中未检测到活动精子。但在食蟹猴中，在性交后30小时检查的26只动物中有14只的黏液中有活动精子。这些精子的活动率、游离精子百分比和游动速度均低于在性交后6小时观察到的精子。通过经宫颈或经子宫抽吸从食蟹猴采集子宫内精子。在经宫颈技术中，在性交后6小时检查的9只动物中有4只成功获取精子，在性交后30小时检查的5只动物中有4只成功获取精子。子宫液中活动精子的百分比很高，为82%±4%，游动速度（86±2微米/秒）高于在宫颈黏液中观察到的速度。约5% - 10%的子宫内精子表现出与大多数哺乳动物中观察到的超激活运动相似的游动动作。这些发现表明，食蟹猴体内精子与宫颈黏液的相互作用比恒河猴的更类似于人类情况。
