Ying Kezhen, Gilmour D James, Zimmerman William B
Department of Chemical and Biological Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD, UK.
Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK.
Int J Mol Sci. 2015 May 19;16(5):11509-21. doi: 10.3390/ijms160511509.
A periodic CO2 dosing strategy for D. salina 19/30 batch culture is proposed. A model of periodic CO2 dosing including dosing time calculation, dosing interval estimation and final chlorophyll yield prediction was established. In experiments, 5% CO2/95% N2 gas was periodically dosed into D. salina culture. Two different gas dosing flow rates were tested. The corresponding dosing time for each flow rate was estimated via the model (10 min·d-1 for 0.7 L·min-1 and 36 min·d-1 for 0.3 L·min-1). Daily pH measurements showed that the pH of these cultures dosed periodically was always kept between 7.5 and 9.5, which highlights that periodic gas supply can maintain a suitable range of pH for microalgal growth without expensive buffers. Notably the culture dosed for set daily intervals was seen to have similar growth to the culture supplied constantly, but with much higher CO2 capture efficiency (11%-18%) compared to continuous dosing (0.25%). It shows great potential for using periodic gas supply to reduce cost, wasted gas and energy use.
提出了一种用于盐生杜氏藻19/30批次培养的周期性二氧化碳投加策略。建立了一个周期性二氧化碳投加模型，包括投加时间计算、投加间隔估计和最终叶绿素产量预测。在实验中，将5%二氧化碳/95%氮气周期性地投加到盐生杜氏藻培养物中。测试了两种不同的气体投加流速。通过该模型估计了每种流速对应的投加时间（0.7 L·min-1时为10 min·d-1，0.3 L·min-1时为36 min·d-1）。每日pH测量表明，这些周期性投加气体的培养物的pH始终保持在7.5至9.5之间，这突出表明周期性气体供应可以在不使用昂贵缓冲剂的情况下为微藻生长维持合适的pH范围。值得注意的是，按设定的每日间隔投加气体的培养物与持续供应气体的培养物具有相似的生长情况，但与连续投加（0.25%）相比，其二氧化碳捕获效率要高得多（11%-18%）。这显示出利用周期性气体供应来降低成本、减少废气和能源使用的巨大潜力。
