Sun Licui, Lu Jiaxi, Wang Qin, Liu Yiqun, Han Feng, Yang Yanhua, Zhang Hongkun, Huang Zhenwu
Wei Sheng Yan Jiu. 2015 Mar;44(2):276-8, 283.
To explore the effects of methylseleninic acid (MeSeA), selenomethionine (SeMet) and methylselenocysteine (MeSeCys) on proliferation, migration and adhesion of HeLa cells.
HeLa cells were cultured and treated with MeSeA, SeMet and MeSeCys for 12 - 72 h respectively. MTT assay, healing assay and in vitro cell Matrigel adhesion assay were used to detect the proliferation, migration and adhesion of HeLa cells.
Compared to the control group, the proliferation of HeLa cells was remarkably inhibited by MeSeA (P <0. 01). The migration of HeLa cells in MeSeA group was inhibited by 34% (P < 0. 05) and 26% (P < 0. 05) in 4 h and 8 h, respectively. However, the migration of HeLa cells with inhibitions of 18% and 13% was in SeMet group in 4 h and 8 h. The inhibitions of HeLa cell migration in MeSeCys group was 28% (P < 0.05) and 5% in 4 h and 8 h, respectively. In addition, the adhesive function of HeLa cells in the MeSeA group, the SeMet group as well as the MeSeCys group were inhibited by 36% (P < 0. 01), 25% and 49% (P < 0. 01).
The proliferation and migration of HeLa cell were effectively inhibited by MeSeA, while the adhesive function of HeLa cell was remarkably inhibited by MeSeCys.
探讨甲基亚硒酸（MeSeA）、硒代蛋氨酸（SeMet）和甲基硒代半胱氨酸（MeSeCys）对人宫颈癌HeLa细胞增殖、迁移和黏附的影响。
培养HeLa细胞，分别用MeSeA、SeMet和MeSeCys处理12 - 72小时。采用MTT法、划痕实验和体外细胞基质胶黏附实验检测HeLa细胞的增殖、迁移和黏附情况。
与对照组相比，MeSeA显著抑制HeLa细胞的增殖（P <0.01）。MeSeA组HeLa细胞在4小时和8小时的迁移分别受到34%（P <0.05）和26%（P <0.05）的抑制。然而，SeMet组HeLa细胞在4小时和8小时的迁移抑制率分别为18%和13%。MeSeCys组HeLa细胞在4小时和8小时的迁移抑制率分别为28%（P <0.05）和5%。此外，MeSeA组、SeMet组和MeSeCys组HeLa细胞的黏附功能分别受到36%（P <0.01）、25%和49%（P <0.01）的抑制。
MeSeA有效抑制HeLa细胞的增殖和迁移，而MeSeCys显著抑制HeLa细胞的黏附功能。
