Chen Liwen, Guan Shihe, Zhou Qiang, Sheng Shouqin, Zhong Fei, Wang Qin
Department of Laboratory Medicine, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China.
Department of Medical Research Center, The Second Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China.
Mol Med Rep. 2015 Sep;12(3):3309-3314. doi: 10.3892/mmr.2015.3796. Epub 2015 May 18.
CD83 is a widely recognized surface marker for mature dendritic cells, which are essential for priming naïve CD4+ T cells into effector cells. However, CD83 is also expressed on activated CD4+ T cells, which remains an enigma in T‑cell mediated immunity. Therefore, the identification of the biological features and regulation of the expression of CD83 on activated CD4+ T cells is important in understanding the function of CD83 in the adaptive immune response. The present study revealed a time‑dependent manner of the expression of CD83 on anti‑CD3/CD28‑stimulated human CD4+ T cells, which is characterized by the maximum expression at day 2 and a significant decrease at day 3. The reduced expression is not a result of a reduced rate of cell proliferation. The activation of interleukin‑2 and secretion of interferon‑γ accumulated progressively from day 1 to 3. Of note, sustained expression of CD83 was observed when CD4+ T cells were induced by transforming growth factor-β to differentiate into CD4+CD25+ forkhead box P3+ regulatory T (iTreg) cells. Confocal immunofluorescence microscopy analysis demonstrated that CD83 was highly co‑localized with CD25 on activated CD4+ T cells. In conclusion, the findings of the present study suggested that the continuous expression of CD83 on activated human CD4+ T cells is correlated with their differentiation into iTreg cells.
CD83是成熟树突状细胞广泛认可的表面标志物，而成熟树突状细胞对于将初始CD4+ T细胞启动分化为效应细胞至关重要。然而，CD83也在活化的CD4+ T细胞上表达，这在T细胞介导的免疫中仍是一个谜。因此，鉴定活化的CD4+ T细胞上CD83的生物学特性及其表达调控，对于理解CD83在适应性免疫应答中的功能很重要。本研究揭示了抗CD3/CD28刺激的人CD4+ T细胞上CD83表达的时间依赖性方式，其特征是在第2天表达最高，在第3天显著下降。表达降低并非细胞增殖速率降低的结果。从第1天到第3天，白细胞介素-2的激活和干扰素-γ的分泌逐渐积累。值得注意的是，当CD4+ T细胞被转化生长因子-β诱导分化为CD4+CD25+叉头框P3+调节性T（iTreg）细胞时，观察到CD83的持续表达。共聚焦免疫荧光显微镜分析表明，CD83在活化的CD4+ T细胞上与CD25高度共定位。总之，本研究结果表明，活化的人CD4+ T细胞上CD83的持续表达与其分化为iTreg细胞相关。
