Pinto Priscila S, Loureiro Ana P, Penna Bruno, Lilenbaum Walter
Fluminense Federal University, Laboratory of Veterinary Bacteriology, Biomedical Institute, 101 Prof. Hernani Mello Street, Niterói, Rio de Janeiro, Brazil.
Fluminense Federal University, Laboratory of Veterinary Bacteriology, Biomedical Institute, 101 Prof. Hernani Mello Street, Niterói, Rio de Janeiro, Brazil.
Acta Trop. 2015 Sep;149:163-7. doi: 10.1016/j.actatropica.2015.05.008. Epub 2015 May 19.
Leptospirosis is a zoonotic disease that occurs worldwide, particularly in tropical countries. In livestock the agent is responsible for reproductive problems such as infertility and abortion. Serogroup Sejroe, particularly serovar Hardjo, prevails in cattle in several regions. The microscopic agglutination test (MAT) is the current method for diagnosing leptospirosis. It has been proposed that the inclusion of local strains could detect a larger set of seroreactive animals. In that context, the aim of the present study was to evaluate if the usage of local strains as antigens increases the sensitivity of the serodiagnosis of bovine leptospirosis. Blood and urine samples were collected from 314 bovines from several herds randomly selected in a slaughterhouse in Rio de Janeiro, Brazil. Serological diagnosis was made with MAT using a 21 reference-strains panel (MAT21). Additionally, 12 local strains (MAT33) were included as antigens. PCR was performed with the urine samples and it was positive on 71 out of 222 samples (31.9%). MAT21 identified as seroreactive 173 (55.1%) out of the 314 animals studied, with Sejroe the most common (38.1%). In MAT33, 204 (65.0%) animals were seroreactive with a significant increase on seroreactivity (9.9%). In conclusion, MAT presented with a significant increase of sensitivity when local strains were used as antigens. Among the local strains, 2013_U152 (KP263062) (serogroup Shermani) and 2013_U280 (KP263069) (serogroup Grippotyphosa) showed to be more antigenic.
钩端螺旋体病是一种在全球范围内发生的人畜共患病，尤其在热带国家较为常见。在牲畜中，该病原体可导致繁殖问题，如不育和流产。 sejroe血清群，特别是hardjo血清型，在多个地区的牛群中占主导地位。显微镜凝集试验（MAT）是目前诊断钩端螺旋体病的方法。有人提出，加入当地菌株可以检测到更多的血清反应阳性动物。在此背景下，本研究的目的是评估使用当地菌株作为抗原是否能提高牛钩端螺旋体病血清诊断的敏感性。从巴西里约热内卢一家屠宰场随机挑选的几个牛群中的314头牛采集血液和尿液样本。使用21种参考菌株面板（MAT21）通过MAT进行血清学诊断。此外，还加入了12种当地菌株（MAT33）作为抗原。对尿液样本进行PCR检测，222份样本中有71份（31.9%）呈阳性。在所研究的314头动物中，MAT21鉴定出173头（55.1%）血清反应阳性，其中sejroe最为常见（38.1%）。在MAT33中，204头（65.0%）动物血清反应阳性，血清反应性显著增加（9.9%）。总之，当使用当地菌株作为抗原时，MAT的敏感性显著提高。在当地菌株中，2013_U152（KP263062）（Shermani血清群）和2013_U280（KP263069）（Grippotyphosa血清群）显示出更强的抗原性。
