用于构建肠道平滑肌的具有排列纤维的正交定向支架。
Orthogonally oriented scaffolds with aligned fibers for engineering intestinal smooth muscle.
作者信息
Kobayashi Masae, Lei Nan Ye, Wang Qianqian, Wu Benjamin M, Dunn James C Y
机构信息
Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Surgery, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Los Angeles, CA 90095, USA.
出版信息
Biomaterials. 2015 Aug;61:75-84. doi: 10.1016/j.biomaterials.2015.05.023. Epub 2015 May 15.
Controlling cellular alignment is critical in engineering intestines with desired structure and function. Although previous studies have examined the directional alignment of cells on the surface (x-y plane) of parallel fibers, quantitative analysis of the cellular alignment inside implanted scaffolds with oriented fibers has not been reported. This study examined the cellular alignment in the x-z and y-z planes of scaffolds made with two layers of orthogonally oriented fibers. The cellular orientation inside implanted scaffolds was evaluated with immunofluorescence. Quantitative analysis of coherency between cell orientation and fiber direction confirmed that cells aligned along the fibers not only on the surface (x-y plane) but also inside the scaffolds (x-z & y-z planes). Our study demonstrated that two layers of orthogonally aligned scaffolds can generate the histological organization of cells similar to that of intestinal circular and longitudinal smooth muscle.
控制细胞排列对于构建具有理想结构和功能的肠道至关重要。尽管先前的研究已经考察了细胞在平行纤维表面（x-y平面）的定向排列，但尚未有关于具有定向纤维的植入支架内部细胞排列的定量分析报道。本研究考察了由两层正交定向纤维制成的支架在x-z和y-z平面上的细胞排列情况。通过免疫荧光评估植入支架内部的细胞取向。对细胞取向与纤维方向之间的一致性进行定量分析证实，细胞不仅在表面（x-y平面）沿纤维排列，而且在支架内部（x-z和y-z平面）也沿纤维排列。我们的研究表明，两层正交排列的支架能够产生与肠道环形和纵向平滑肌相似的细胞组织学结构。
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