Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PEI, Canada C1A 4P3.
Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PEI, Canada C1A 4P3; Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North 4442, New Zealand.
Talanta. 2015 Sep 1;142:110-9. doi: 10.1016/j.talanta.2015.04.010. Epub 2015 Apr 23.
Immunoglobulin G (IgG) is crucial for the protection of the host from invasive pathogens. Due to its importance for human health, tools that enable the monitoring of IgG levels are highly desired. Consequently there is a need for methods to determine the IgG concentration that are simple, rapid, and inexpensive. This work explored the potential of attenuated total reflectance (ATR) infrared spectroscopy as a method to determine IgG concentrations in human serum samples. Venous blood samples were collected from adults and children, and from the umbilical cord of newborns. The serum was harvested and tested using ATR infrared spectroscopy. Partial least squares (PLS) regression provided the basis to develop the new analytical methods. Three PLS calibrations were determined: one for the combined set of the venous and umbilical cord serum samples, the second for only the umbilical cord samples, and the third for only the venous samples. The number of PLS factors was chosen by critical evaluation of Monte Carlo-based cross validation results. The predictive performance for each PLS calibration was evaluated using the Pearson correlation coefficient, scatter plot and Bland-Altman plot, and percent deviations for independent prediction sets. The repeatability was evaluated by standard deviation and relative standard deviation. The results showed that ATR infrared spectroscopy is potentially a simple, quick, and inexpensive method to measure IgG concentrations in human serum samples. The results also showed that it is possible to build a united calibration curve for the umbilical cord and the venous samples.
免疫球蛋白 G（IgG）对于宿主免受入侵性病原体的侵害至关重要。由于其对人类健康的重要性，因此非常需要能够监测 IgG 水平的工具。因此，需要开发简单、快速且廉价的方法来确定 IgG 浓度。本研究探索了衰减全反射（ATR）红外光谱作为一种测定人血清样品中 IgG 浓度的方法的潜力。从成人和儿童以及新生儿的脐带中采集静脉血样本。采集血清并使用 ATR 红外光谱进行测试。偏最小二乘（PLS）回归为开发新的分析方法提供了基础。确定了三个 PLS 校准：一个用于静脉和脐带血清样品的组合集，第二个仅用于脐带样品，第三个仅用于静脉样品。通过基于蒙特卡罗的交叉验证结果的临界评估选择 PLS 因子的数量。使用 Pearson 相关系数、散点图和 Bland-Altman 图以及独立预测集的百分比偏差评估每个 PLS 校准的预测性能。通过标准差和相对标准差评估重复性。结果表明，ATR 红外光谱是一种简单、快速且廉价的方法，可用于测量人血清样品中的 IgG 浓度。结果还表明，有可能为脐带和静脉样本建立联合校准曲线。
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