Ayyub Asima, Saleem Mahjabeen, Musharraf Syed Ghulam, Naz Mamoona, Tariq Asma, Hashmi Naghma
Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore 54590, Pakistan.
Hussain Ebrahim Jamal Research Institute, University of Karachi, Karachi 75270, Pakistan.
Mol Med Rep. 2015 Sep;12(3):3755-3762. doi: 10.3892/mmr.2015.3822. Epub 2015 May 22.
Lung cancer is the major contributor to overall cancer-related mortality. Biomarkers are important in early detection and prognosis, in addition to developing treatment regimes, which may improve the patient survival rates. Biomarkers may also assist in investigating the in depth metabolic pathways and in establishing a set of therapeutic agents leading to early detection of the disease. The present study was designed to identify and confirm a lung cancer protein biomarker and to correlate the differential expression of the protein to a particular histological disease type. A total of 100 lung cancer patients and 50 healthy controls were included in the present study and were categorized into the two main histological types of lung cancer; non‑small cell lung cancer (NSCLC; n=88) and small cell lung cancer (SCLC; n=12). NSCLC was further subclassified into three histological types; adenocarcinoma (n=34), squamous cell carcinoma (n=48) and large cell carcinoma (n=6). The patient and control serum samples underwent sodium dodecyl sulphate polyacrylamide gel electrophoresis characterization followed by two‑dimensional gel electrophoresis. Following mass spectrometry, human haptoglobin was identified with a mass of ~42‑46 kDa and an isoelectric point (pI) of ~5.5‑6.2. The experimental mass of the protein was found to be 45.8 kDa with a pI of 6.13. The matrix‑assisted laser desorption/ionization time‑of‑flight/time‑of‑flight data exhibited spectral peaks of 1146.134, 1724.191, 1345.339 and 2210.319 m/z and Mascot search analysis identified these peaks as haptoglobin (accession no. P00738; Mascot score 87; sequence coverage 23%). This protein was significantly overexpressed in squamous cell carcinoma and adenocarcinoma, as compared with the control. The present study described differentially expressed human haptoglobin as a lung cancer serum protein biomarker, which may serve as a diagnostic and therapeutic target and set a standard criteria for the evaluation of histological types of lung cancer compared with other disease types.
肺癌是导致癌症相关总体死亡率的主要因素。生物标志物在早期检测和预后评估中很重要，此外在制定治疗方案方面也很重要，这可能会提高患者的生存率。生物标志物还可能有助于深入研究代谢途径，并确定一系列能够实现疾病早期检测的治疗药物。本研究旨在鉴定和确认一种肺癌蛋白质生物标志物，并将该蛋白质的差异表达与特定的组织学疾病类型相关联。本研究共纳入了100例肺癌患者和50例健康对照，并将其分为肺癌的两种主要组织学类型；非小细胞肺癌（NSCLC；n = 88）和小细胞肺癌（SCLC；n = 12）。NSCLC进一步细分为三种组织学类型；腺癌（n = 34）、鳞状细胞癌（n = 48）和大细胞癌（n = 6）。对患者和对照的血清样本进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳表征，随后进行二维凝胶电泳。经过质谱分析，鉴定出人类触珠蛋白，其分子量约为42 - 46 kDa，等电点（pI）约为5.5 - 6.2。该蛋白质的实验分子量为45.8 kDa，pI为6.13。基质辅助激光解吸/电离飞行时间/飞行时间数据显示出1146.134、1724.191、1345.339和2210.319 m/z的光谱峰，Mascot搜索分析将这些峰鉴定为触珠蛋白（登录号P00738；Mascot得分87；序列覆盖率23%）。与对照相比，该蛋白质在鳞状细胞癌和腺癌中显著过表达。本研究将差异表达的人类触珠蛋白描述为一种肺癌血清蛋白质生物标志物，它可能作为诊断和治疗靶点，并为与其他疾病类型相比评估肺癌组织学类型设定标准。
