Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, CP 6171, Campinas, SP CEP 13081-970, Brazil.
Laboratory of Biological Processes, Department of Hydraulics and Sanitation, Engineering School of São Carlos - University of São Paulo (EESC - USP) Campus II, São Carlos, SP CEP 13563-120, Brazil.
Bioresour Technol. 2015 Sep;192:37-45. doi: 10.1016/j.biortech.2015.05.050. Epub 2015 May 20.
The objective of this study was to evaluate the removal of linear alkylbenzene sulfonate (LAS) from commercial laundry wastewater using an expanded granular sludge bed (EGSB) reactor with two specific LAS loading rates (SLLRs), 1.0 and 2.7 mg LAS gVS(-1)d (-1). The biomass was characterized using denaturing gradient gel electrophoresis (DGGE) and 16S Ion Tag sequencing. Higher LAS removal (92.9%) was observed in association with an SLLR of 1.0 mg LAS gVS(-1) d(-1) than with an SLLR of 2.7 mg LAS gVS(-1) d(-1) (58.6%). A relationship between the S(-2) concentration in the effluent and the surfactant removal efficiency was observed. This result is indicative of the inhibition of LAS-removing microbiota at S(-2) concentrations greater than 20 mg SL(-1). By using DGGE, microbial stratification was observed in the reactor in association with granule size, even though the reactor is considered to be a completely mixed regime. The RDP-classifier identified 175 genera, 33 of which were related to LAS degradation.
本研究旨在采用膨胀颗粒污泥床（EGSB）反应器，以两种特定的线性烷基苯磺酸钠（LAS）负荷率（SLLR），即 1.0 和 2.7 mg LAS gVS(-1) d (-1)，评估其去除商用洗衣废水中 LAS 的效果。采用变性梯度凝胶电泳（DGGE）和 16S Ion Tag 测序对生物量进行了表征。与 SLLR 为 2.7 mg LAS gVS(-1) d(-1)相比，SLLR 为 1.0 mg LAS gVS(-1) d(-1)时，LAS 的去除率更高（92.9%）。在出水中 S(-2)浓度与表面活性剂去除效率之间观察到了一种关系。这一结果表明，在 S(-2)浓度大于 20 mg SL(-1)时，LAS 去除微生物会受到抑制。通过使用 DGGE，即使反应器被认为是完全混合的，也观察到了与颗粒大小有关的微生物分层现象。RDP 分类器确定了 175 个属，其中 33 个与 LAS 降解有关。
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