Kim Myung Kyum, Back Chang-Gi, Jung Hee-Young, Srinivasan Sathiyaraj
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
National Institute of Horticultural & Herbal Science, RDA, 100 Nongsaenyeong-ro, Iseo-myeon, Wanji-gun, Jeollabuk-do, Republic of Korea.
J Biotechnol. 2015 Aug 20;208:11-2. doi: 10.1016/j.jbiotec.2015.05.004. Epub 2015 May 22.
Spirosoma radiotolerans is a Gram-negative, short rod-shaped and gamma-radiation-resistant bacterium isolated from rice field in South Korea (GPS; 37°34'30″N, 127°00'30″E). The complete genome of S. radiotolerans consists of a chromosome (7,029,352bp). From the genome sequence database, we have identified the cluster of genes responsible for DNA recovery from ionizing radiation. The key enzymes for the nucleotide excision repair (NER) were investigated and were identified, suggesting that S. radiotolerans DG5A use (NER) pathways for efficient removal of pyrimidine dimers which are the most abundant type of UV-induced damage. Complete genome information enables further studies on the DNA repair mechanisms during the ionizing radiation.
耐辐射螺菌是一种革兰氏阴性、短杆状且耐γ辐射的细菌,从韩国稻田(GPS坐标:北纬37°34'30″,东经127°00'30″)分离得到。耐辐射螺菌的完整基因组由一条染色体(7,029,352bp)组成。从基因组序列数据库中,我们鉴定出了负责从电离辐射中恢复DNA的基因簇。对核苷酸切除修复(NER)的关键酶进行了研究并得以鉴定,这表明耐辐射螺菌DG5A利用(NER)途径来有效去除嘧啶二聚体,嘧啶二聚体是紫外线诱导损伤中最常见的类型。完整的基因组信息有助于进一步研究电离辐射期间的DNA修复机制。