Sousa Louisa M A, Carneiro Matheus B H, Dos Santos Liliane M, Natale Caio Cotta, Resende Magda E, Mosser David M, Vieira Leda Q
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil; Maryland Pathogen Research Institute, University of Maryland, College Park, MD, USA.
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Cytokine. 2015 Aug;74(2):327-30. doi: 10.1016/j.cyto.2015.01.021. Epub 2015 May 23.
We evaluated the role of IL-18 during Leishmania amazonensis infection in C57BL/6 mice, using IL-18KO mice. We showed that IL-18 is involved in susceptibility to L. amazonensis, since IL-18KO mice presented reduced lesions and parasite loads. Because macrophages are the host cells of the parasite, we investigated if macrophages were involved in IL-18-mediated susceptibility to L. amazonensis. We showed that macrophages obtained from WT or IL-18KO responded similarly to L. amazonensis infection. Moreover, we showed that C57BL/6 macrophages do not respond to IL-18, since they do not express IL-18R. Therefore, macrophages are not involved in IL-18-mediated susceptibility to L. amazonensis.
我们使用白细胞介素-18基因敲除(IL-18KO)小鼠评估了白细胞介素-18(IL-18)在亚马逊利什曼原虫感染C57BL/6小鼠过程中的作用。我们发现IL-18与对亚马逊利什曼原虫的易感性有关,因为IL-18KO小鼠的病变和寄生虫负荷有所减轻。由于巨噬细胞是该寄生虫的宿主细胞,我们研究了巨噬细胞是否参与IL-18介导的对亚马逊利什曼原虫的易感性。我们发现,从野生型(WT)或IL-18KO小鼠获得的巨噬细胞对亚马逊利什曼原虫感染的反应相似。此外,我们还发现C57BL/6巨噬细胞对IL-18无反应,因为它们不表达IL-18受体。因此,巨噬细胞不参与IL-18介导的对亚马逊利什曼原虫的易感性。