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使用共聚焦显微镜对器械操作后粗糙钛表面的塑料残留进行定量分析,并评估去除效果。

Use of confocal microscopy for quantification of plastic remnants on rough titanium after instrumentation and evaluation of efficacy of removal.

作者信息

Yang Seung-Min, Park Jun-Beom, Ko Youngkyung

出版信息

Int J Oral Maxillofac Implants. 2015 May-Jun;30(3):519-25. doi: 10.11607/jomi.3500.

Abstract

PURPOSE

Many experts have proposed the treatment of titanium surfaces with plastic or nonmetal tips to prevent damage to implant surfaces by metal instruments during professional cleaning. It is also known that any material that is softer than titanium may leave remnants of itself on the treated surface. The aim of this study was to quantify the surface area covered by plastic remnants after instrumentation with various plastic instruments and to evaluate the efficacy of the removal of these remnants by confocal microscopy.

MATERIALS AND METHODS

Twenty-seven rough-surfaced titanium disks were used for quantification of plastic remnants. Nine disks each were cleaned with (1) a plastic tip on ultrasonic scaler A, (2) a carbon tip on ultrasonic scaler B, or (3) a plastic curette. Three disks from each group were cleaned with a three-way syringe for 10 seconds, and three other disks were cleaned with 0.2% chlorhexidine-soaked cotton pellets. Images were obtained at random areas on each disk with a confocal microscope, and a digital image analysis program was used to quantify the area with autofluorescence.

RESULTS

Approximately 10% to 20% of the surface was covered with plastic remnants of the instrument, irrespective of the instrument used. These remnants were not removed with a three-way syringe or chlorhexidine-soaked cotton pellets.

CONCLUSION

Plastic remnants remained after instrumentation, regardless of the instrument used. Moreover, the study highlighted the difficulty of removing all remnants with water spray or a chlorhexidine-soaked cotton pellet. This study suggests that confocal microscopy is a reproducible method for quantification that may be applied for future comparisons of methods to remove plastic residue. Further research is warranted to evaluate the biologic effects of plastic remnants.

摘要

目的

许多专家提议使用塑料或非金属尖端处理钛表面,以防止在专业清洁过程中金属器械对种植体表面造成损伤。众所周知,任何比钛软的材料都可能在处理过的表面留下自身的残余物。本研究的目的是量化使用各种塑料器械处理后塑料残余物覆盖的表面积,并通过共聚焦显微镜评估去除这些残余物的效果。

材料与方法

使用27个粗糙表面的钛盘来量化塑料残余物。每组9个盘分别用以下方法清洁:(1)超声洁牙器A上的塑料尖端,(2)超声洁牙器B上的碳尖端,或(3)塑料刮匙。每组中的三个盘用三用注射器清洁10秒,另外三个盘用0.2%氯己定浸泡的棉球清洁。用共聚焦显微镜在每个盘的随机区域获取图像,并使用数字图像分析程序量化自发荧光区域。

结果

无论使用何种器械,约10%至20%的表面被器械的塑料残余物覆盖。这些残余物不能用三用注射器或氯己定浸泡的棉球去除。

结论

无论使用何种器械,处理后都会残留塑料残余物。此外,该研究突出了用水喷雾或氯己定浸泡的棉球去除所有残余物的困难。本研究表明,共聚焦显微镜是一种可重复的量化方法,可用于未来去除塑料残留物方法的比较。有必要进一步研究以评估塑料残余物的生物学效应。

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