Lizasoain A, Tort L F L, García M, Gómez M M, Leite J P G, Miagostovich M P, Cristina J, Colina R, Victoria M
Laboratory of Molecular Virology, Department of Biological Sciences, Regional Norte, CENUR Noroeste, Universidad de la República, Salto, Uruguay.
Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
J Appl Microbiol. 2015 Sep;119(3):859-67. doi: 10.1111/jam.12856. Epub 2015 Jul 8.
The aim of this study was to investigate the presence of the recently identified human astrovirus (HAstV) and to increase the knowledge of the molecular epidemiology of classical HAstV detected in Uruguay.
Recently identified and classical HAstV genotypes were investigated by RT-PCR targeting the ORF1b and ORF2 genome regions in 20 samples obtained between September 2011 and April 2013 in two cities of the eastern region of Uruguay. Four of 20 samples (20%) were identified as MLB-1 genotype and it was found a new MLB-1 classification through the segregation of the worldwide reported MLB-1 strains in two genetic lineages proposed and named: MLB-1a and MLB-1b. Fourteen (70%) samples were positive for classical HAstV and 12 of them were successfully sequenced and genotyped as: HAstV-1 (n = 10), HAstV-2 and HAstV-5 (one sample each).
These results constitute the first report in the Latin American region concerning the molecular detection and characterization of MLB-1 HAstV strains in environmental samples.
This study highlights the benefits of an environmental surveillance to study emerging enteric viruses circulating in human societies.
本研究旨在调查最近鉴定出的人类星状病毒（HAstV）的存在情况，并增进对在乌拉圭检测到的经典HAstV分子流行病学的了解。
通过逆转录聚合酶链反应（RT-PCR）针对ORF1b和ORF2基因组区域，对2011年9月至2013年4月期间在乌拉圭东部地区两个城市采集的20份样本中的最近鉴定出的和经典的HAstV基因型进行了调查。20份样本中有4份（20%）被鉴定为MLB-1基因型，并且通过将全球报告的MLB-1毒株分为两个提议并命名的遗传谱系：MLB-1a和MLB-1b，发现了一种新的MLB-1分类。14份（70%）样本的经典HAstV呈阳性，其中12份成功测序并进行基因分型，分别为：HAstV-1（n = 10）、HAstV-2和HAstV-5（各1份样本）。
这些结果构成了拉丁美洲地区关于环境样本中MLB-1 HAstV毒株分子检测和特征描述的首份报告。
本研究突出了环境监测对于研究在人类社会中传播的新兴肠道病毒的益处。
