Iwatani Y, Amino N, Miyai K
Rinsho Byori. 1989 Oct;37(10):1089-95.
Flow cytometric analysis for lymphocyte subsets provides two kinds of data: proportion of fluorescence-positive cells (lymphocyte subsets) and the fluorescence intensity. For proportional analysis of lymphocyte subsets, lymphocytes are fractionated with a conventional gate window in scatter analysis, but if activated or enlarged lymphocytes are examined, lymphocytes should be fractionated with an extended gate window. On the other hand, fluorescence intensity of a lymphocyte subset, which represents density of cell surface antigen detected by fluorescence-conjugated specific antibody, is not measured in routine tests yet. However, data on fluorescence intensity are often more valuable than the proportions of lymphocyte subsets: e.g., decrease in fluorescence intensity, but not in proportion, of CD8+ cells in active Graves' disease, and marked decrease in fluorescence intensity of OKT4+ cells in a carrier of familial OKT 4 epitope deficiency. Furthermore, two-color flow cytometry has enabled to measure more valuable, classified subsets of lymphocytes than single color flow cytometry. For example, measurement of peripheral CD5 (Leu-1)+ B cells is very useful for diagnosis of Graves' disease and its differentiation from destruction-induced thyrotoxicosis. In conclusion, we showed that measurement of fluorescence intensity of lymphocyte subsets and two-color flow cytometry are useful for laboratory tests of lymphocyte subsets.
荧光阳性细胞（淋巴细胞亚群）的比例和荧光强度。对于淋巴细胞亚群的比例分析，在散射分析中用传统的门控窗对淋巴细胞进行分群，但如果检测活化或增大的淋巴细胞，则应用扩展的门控窗对淋巴细胞进行分群。另一方面，淋巴细胞亚群的荧光强度代表通过荧光偶联特异性抗体检测到的细胞表面抗原密度，目前常规检测中尚未进行测量。然而，荧光强度数据通常比淋巴细胞亚群的比例更有价值：例如，在活动性格雷夫斯病中CD8 +细胞的荧光强度降低，但比例未降低，以及在家族性OKT 4表位缺陷携带者中OKT4 +细胞的荧光强度显著降低。此外，双色流式细胞术能够比单色流式细胞术测量更有价值的、分类的淋巴细胞亚群。例如，外周血CD5（Leu-1）+ B细胞的检测对于格雷夫斯病的诊断及其与破坏性甲状腺毒症的鉴别非常有用。总之，我们表明淋巴细胞亚群荧光强度的测量和双色流式细胞术对于淋巴细胞亚群的实验室检测是有用的。
