采用多重实时聚合酶链反应检测保加利亚不育男性中的沙眼衣原体、解脲脲原体和人型支原体。

Detection of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis in infertile Bulgarian men with multiplex real-time polymerase chain reaction.

作者信息

Ouzounova-Raykova Vessela, Rangelov Simeon, Ouzounova Iordanka, Mitov Ivan

机构信息

Department of Medical Microbiology, Medical University-Sofia, Sofia, Bulgaria.

Department of Andrology, University Hospital 'Tsaritsa Yoanna', Medical University-Sofia, Sofia, Bulgaria.

出版信息

APMIS. 2015 Jul;123(7):586-8. doi: 10.1111/apm.12391. Epub 2015 May 22.

DOI:10.1111/apm.12391

PMID:26010780

Abstract

The effect of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis over the sperm quality is still unclear. The aim of this study was to determine their prevalence in infertile Bulgarian men. A total of 281 men were examined by applying mRT-PCR. The registered prevalence was as follows: C. trachomatis - 13.9%, U. urealyticum - 19.2%, M. hominis - 9.9%. Co-infection was established in eight swabs. This first in Bulgaria to study for detection of chlamydia and mycoplasmas in infertile men by mRT-PCR demonstrates higher prevalence of the tested microorganisms in the infertile group toward the control one.

摘要

沙眼衣原体、解脲脲原体和人型支原体对精子质量的影响仍不清楚。本研究的目的是确定它们在保加利亚不育男性中的流行率。通过应用mRT-PCR对281名男性进行了检查。登记的流行率如下：沙眼衣原体-13.9%，解脲脲原体-19.2%，人型支原体-9.9%。在8份拭子中发现了合并感染。这是保加利亚首次通过mRT-PCR研究不育男性中衣原体和支原体的检测，结果表明不育组中所检测微生物的流行率高于对照组。