疑似败血性关节炎犬的滑液培养与16S rRNA聚合酶链反应的比较
Comparison of synovial fluid culture and 16S rRNA PCR in dogs with suspected septic arthritis.
作者信息
Scharf V F, Lewis D D, Wellehan J F, Wamsley H L, Richardson R
机构信息
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Department of Physiologic Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
出版信息
Aust Vet J. 2015 Jun;93(6):204-7. doi: 10.1111/avj.12329.
OBJECTIVE
To prospectively compare the sensitivity and specificity of 16S rRNA PCR with culture for identifying the causative organism in synovial fluid obtained from dogs with suspected septic arthritis.
METHODS
Synovial fluid cytology, PCR analysis and aerobic, anaerobic and Mycoplasma culture of samples from the affected joints of 18 dogs presenting with suspected septic arthritis were performed. Synovial fluid samples from the corresponding contralateral joints of 7 dogs were also analysed as negative controls.
RESULTS
There was no significant difference between the sensitivity of bacterial detection via culture (63.2%) versus PCR (73.7%) of synovial fluid (P=0.728) or between culture and combined PCR and culture (89.5%) of synovial fluid (P=0.124). The specificity of PCR (42.9%) was significantly lower than culture specificity (100%) (P=0.07).
CONCLUSION
Although 16S PCR may hold potential as an ancillary diagnostic test for identifying the causative organism in dogs with septic arthritis, our study failed to demonstrate improved accuracy compared with traditional synovial fluid culture.
目的
前瞻性比较16S rRNA聚合酶链反应（PCR）与培养法在鉴定疑似脓毒性关节炎犬关节液中致病微生物方面的敏感性和特异性。
方法
对18只疑似脓毒性关节炎犬患关节的样本进行了关节液细胞学检查、PCR分析以及需氧、厌氧和支原体培养。还对7只犬相应对侧关节的关节液样本作为阴性对照进行了分析。
结果
通过关节液培养（63.2%）与PCR（73.7%）检测细菌的敏感性之间无显著差异（P = 0.728），关节液培养与PCR和培养联合检测（89.5%）之间也无显著差异（P = 0.124）。PCR的特异性（42.9%）显著低于培养法的特异性（100%）（P = 0.07）。
结论
尽管16S PCR作为鉴定脓毒性关节炎犬致病微生物的辅助诊断试验可能具有潜力，但我们的研究未能证明其与传统关节液培养相比准确性有所提高。
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