Saeki H, Ishii T
Nihon Juigaku Zasshi. 1989 Dec;51(6):1173-8. doi: 10.1292/jvms1939.51.1173.
A series of experiments were undertaken to determine the most effective route of immunization with a mixture of killed Babesia rodhaini antigen (S antigen) and formalin-fixed Corynebacterium parvum (Propionibacterium acnes) bacterin (CPB) against challenge infection with B. rodhaini 3 weeks later. The mice pretreated with S antigen and CPB mixture intraperitoneally, but not intramuscularly, were significantly resistant to intraperitoneal (IP) or intravenous (IV) challenge with 10(6) organisms. The survival rates were 70.0 (IP challenge) and 60.0% (IV challenge) respectively. Fairly protective activities were equally produced in mice intravenously pretreated with S antigen and CPB with survival rates of 60.0% against IV challenge, but 30% against IP. These results indicated that the IP injection of S antigen and CPB mixture is desirable route for immunization against subsequent IP or IV challenge with B. rodhaini. On the other hand, lower protective effect was reconfirmed in the mice treated with S antigen and Freund's Complete adjuvant, regardless of immunization routes in the additional experiment. The survival rates were 33.3, 14.3 and 11.8% in the intraperitoneally, intramuscularly and subcutaneously-treated mice respectively against IP challenge with 10(6) organisms.
进行了一系列实验，以确定用灭活的罗得西亚巴贝斯虫抗原（S抗原）和福尔马林固定的微小棒状杆菌（痤疮丙酸杆菌）菌苗（CPB）混合物进行免疫的最有效途径，以抵抗3周后罗得西亚巴贝斯虫的攻击感染。用S抗原和CPB混合物进行腹腔注射而非肌肉注射预处理的小鼠，对10(6)个生物体的腹腔内（IP）或静脉内（IV）攻击具有显著抗性。存活率分别为70.0%（IP攻击）和60.0%（IV攻击）。用S抗原和CPB进行静脉预处理的小鼠也产生了相当的保护活性，对IV攻击的存活率为60.0%，但对IP攻击为30%。这些结果表明，腹腔注射S抗原和CPB混合物是针对随后罗得西亚巴贝斯虫的IP或IV攻击进行免疫的理想途径。另一方面，在额外实验中，无论免疫途径如何，用S抗原和弗氏完全佐剂处理的小鼠的保护作用均较低。腹腔内、肌肉内和皮下处理的小鼠分别对10(6)个生物体的IP攻击的存活率为33.3%、14.3%和11.8%。
