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[不同培养条件下巨大芽孢杆菌细胞中的金属含量]

[Metal content in Bacillus megaterium cells under different cultivation conditions].

作者信息

Sakharova Z V, Shaforostova L F, Kochetkov G A

出版信息

Mikrobiologiia. 1978 Jan-Feb;47(1):61-6.

PMID:26013
Abstract

The content of K, Na, Mg, Fe, Al, Ba, Sr and Cu was studied in the cells of a chemostal culture of Bacillus megaterium whose growth was limited with citrate, the growth rates being D=mu=0.2, 0.4 and 0.7 hr(-1). The growth was affected also by acid and alkaline values of pH. The content of metals in the cells could change tenfold, depending on their physiological state. The content of Ca, Mg and Fe increased with the growth rate while that of K remained constant. The content of metals changed even more sharply, depending on pH. In the alkaline medium, the content of K, Na, Mg, Ca and Fe decreased, in contrast to that of Al, Ba, Sr and Cu, probably as a result of damaged transport.

摘要

研究了巨大芽孢杆菌恒化培养细胞中钾(K)、钠(Na)、镁(Mg)、铁(Fe)、铝(Al)、钡(Ba)、锶(Sr)和铜(Cu)的含量,该培养中细胞生长受柠檬酸盐限制,生长速率分别为D = μ = 0.2、0.4和0.7 h⁻¹。生长还受到pH值的酸性和碱性影响。细胞中金属的含量可根据其生理状态变化十倍。钙(Ca)、镁(Mg)和铁(Fe)的含量随生长速率增加,而钾(K)的含量保持恒定。金属含量甚至因pH值而变化得更剧烈。在碱性培养基中,钾(K)、钠(Na)、镁(Mg)、钙(Ca)和铁(Fe)的含量下降,与铝(Al)、钡(Ba)、锶(Sr)和铜(Cu)相反,这可能是运输受损的结果。

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