Jia Xiaojuan, Bi Yuhai, Li Jing, Xie Qing, Yang Hanchun, Liu Wenjun
1] Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, China [2] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Sci Rep. 2015 May 27;5:10651. doi: 10.1038/srep10651.
Porcine reproductive and respiratory syndrome (PRRS) has caused large economic losses in the swine industry in recent years. Current PRRS vaccines fail to effectively prevent and control this disease. Consequently, there is a need to develop new antiviral strategies. MicroRNAs play critical roles in intricate host-pathogen interaction networks, but the involvement of miRNAs during PRRS virus (PRRSV) infection is not well understood. In this study, pretreatment with miR-26a induced a significant inhibition of PRRSV replication and remission of the cytopathic effect in MARC-145 cells, and this antiviral effect was sustained for at least 120 h. Luciferase reporter analysis showed that the PRRSV genome was not the target of miRNA-26a. Instead, RNA-seq analysis demonstrated that miR-26a significantly up-regulated innate anti-viral responses, including activating the type I interferon (IFN) signaling pathway and promoting the production of IFN-stimulated genes. These findings suggest that delivery of miR-26a may provide a potential strategy for anti-PRRSV therapies.
近年来，猪繁殖与呼吸综合征（PRRS）给养猪业造成了巨大的经济损失。目前的PRRS疫苗无法有效预防和控制这种疾病。因此，有必要开发新的抗病毒策略。微小RNA（MicroRNAs）在复杂的宿主-病原体相互作用网络中发挥着关键作用，但miRNA在猪繁殖与呼吸综合征病毒（PRRSV）感染过程中的作用尚不清楚。在本研究中，用miR-26a预处理可显著抑制MARC-145细胞中PRRSV的复制，并减轻细胞病变效应，且这种抗病毒作用至少持续120小时。荧光素酶报告基因分析表明，PRRSV基因组不是miRNA-26a的靶标。相反，RNA测序分析表明，miR-26a显著上调了先天性抗病毒反应，包括激活I型干扰素（IFN）信号通路和促进IFN刺激基因的产生。这些发现表明，递送miR-26a可能为抗PRRSV治疗提供一种潜在策略。
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