Liu Wei, Sha Xiangyin, Wen Ye, Zhao Wenting, Luo Wei, Hua Zanmei
Eye Sci. 2014 Dec;29(4):214-8.
To evaluate the effect of Avastin on human pterygium fibroblast migration and invasiveness.
VEGF secretion was compared between human pterygium fibroblasts and conjunctival fibroblasts by measuring VEGF-A by ELISA. The influence of Avastin on HPF migration and invasiveness was observed by wound scratch and Transwell migration assays. The expression of p-ERK1/2 and p-FAK was analyzed by western blotting.
(1)VEGF was secreted in higher amounts by human pterygium fibroblasts than by conjunctival fibroblasts. (2) Avastin treatment decreased HPF migration and invasion. (3) Avastin significantly decreased the expression of p-ERK1/2 and p-FAK in human pterygium fibroblasts.
Avastin can inhibit migration and invasion of HPFs by decreasing the expression of p-ERK1/2 and p-FAK.
评估阿瓦斯汀对人翼状胬肉成纤维细胞迁移和侵袭能力的影响。
通过酶联免疫吸附测定法（ELISA）检测血管内皮生长因子-A（VEGF-A），比较人翼状胬肉成纤维细胞与结膜成纤维细胞之间VEGF的分泌情况。采用划痕实验和Transwell迁移实验观察阿瓦斯汀对人翼状胬肉成纤维细胞（HPF）迁移和侵袭能力的影响。通过蛋白质免疫印迹法分析磷酸化细胞外信号调节激酶1/2（p-ERK1/2）和磷酸化黏着斑激酶（p-FAK）的表达情况。
（1）人翼状胬肉成纤维细胞分泌的VEGF量高于结膜成纤维细胞。（2）阿瓦斯汀处理可降低HPF的迁移和侵袭能力。（3）阿瓦斯汀可显著降低人翼状胬肉成纤维细胞中p-ERK1/2和p-FAK的表达。
阿瓦斯汀可通过降低p-ERK1/2和p-FAK的表达来抑制HPF的迁移和侵袭。
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