Du Yan, Li Li-nan, Fang Bu-wu
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2015 Jan;31(1):14-7.
To investigate the effects of Artesunate(Art) on the LX-2 cell.
The cultured hepatic stellate cells were divided into control group and Art-treated groups with 250,350,450 µmol/L. The rate of cellular proliferation was detected by MIT assay, the content of ceramide (Cer)was determined by HPLC method, the content of hydroxyproline (Hyp) was determined by enzyme digestion method, the expressions of PPAR-γ, p53 and Caspase 3 were detected by Western blot.
Compared with control group, IX-2 treated with Art were inhibited in a concentration-dependent manner(P < 0.01). Art could significantly increase the content of cerarnide in LX-2 ( P <0.01), and the content of Hyp was significantly decreased (P <0.05, P <0.01). The expressions of PPAR-γ, p53 and Caspase 3 were increased compared with that of control group(P < 0.01).
Artesunate could inhibit the proliferation and induce apoptosis of hepatic stellate cells through upregulating ceramide.
研究青蒿琥酯（Art）对LX - 2细胞的作用。
将培养的肝星状细胞分为对照组和250、350、450 μmol/L青蒿琥酯处理组。采用MTT法检测细胞增殖率，用高效液相色谱法测定神经酰胺（Cer）含量，用酶消化法测定羟脯氨酸（Hyp）含量，用蛋白质免疫印迹法检测PPAR - γ、p53和Caspase 3的表达。
与对照组相比，青蒿琥酯处理的LX - 2细胞增殖受到浓度依赖性抑制（P < 0.01）。青蒿琥酯可显著增加LX - 2细胞中神经酰胺含量（P < 0.01），羟脯氨酸含量显著降低（P < 0.05，P < 0.01）。与对照组相比，PPAR - γ、p53和Caspase 3的表达增加（P < 0.01）。
青蒿琥酯可通过上调神经酰胺抑制肝星状细胞增殖并诱导其凋亡。
