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通过固态发酵将黄酒糟转化为高蛋白酵母培养物。

Conversion of yellow wine lees into high-protein yeast culture by solid-state fermentation.

作者信息

Hu Yuanliang, Pan Lina, Dun Yaohao, Peng Nan, Liang Yunxiang, Zhao Shumiao

机构信息

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University , Wuhan 430070 , P.R. China ; College of Life Sciences, Hubei Normal University , Huangshi , Hubei 435002 , P.R. China.

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University , Wuhan 430070 , P.R. China.

出版信息

Biotechnol Biotechnol Equip. 2014 Sep 3;28(5):843-849. doi: 10.1080/13102818.2014.962407. Epub 2014 Oct 22.

Abstract

This study is focussed on the possibility of producing a yeast culture with yellow wine lees as a substrate by solid-state fermentation (SSF). Results showed that a yeast count of 1.58 × 10 CFU/g was achieved by signal factor and orthogonal experiments. After fermentation, the starch content in the yeast culture reduced from 32.2% ± 0.5% to 7.5% ± 0.2%, and the contents of crude protein and peptide increased from 36.1% ± 0.8% to 48.0% ± 1.0% and 3.9% ± 0.2% to 7.2% ± 0.4%, respectively. Additionally, large amounts of short peptides and free amino acids were detected by fast protein liquid chromatography (FPLC). These results suggest that yellow wine lees are a suitable substrate for the production of yeast cultures. It can serve as a growth-promoting factor and help reduce the shortage of protein feed in the animal industry. This research provides a potential way for the utilization of agro-industrial residues.

摘要

本研究聚焦于以黄酒糟为底物通过固态发酵(SSF)生产酵母培养物的可能性。结果表明,通过单因素和正交试验,酵母培养物中的酵母计数达到了1.58×10 CFU/g。发酵后,酵母培养物中的淀粉含量从32.2%±0.5%降至7.5%±0.2%,粗蛋白和肽的含量分别从36.1%±0.8%增至48.0%±1.0%以及从3.9%±0.2%增至7.2%±0.4%。此外,通过快速蛋白质液相色谱(FPLC)检测到大量的短肽和游离氨基酸。这些结果表明,黄酒糟是生产酵母培养物的合适底物。它可作为一种生长促进因子,有助于减少畜牧业中蛋白质饲料的短缺。本研究为农业工业废弃物的利用提供了一条潜在途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/909c/4433825/de9f02eb482d/tbeq-28-843-g001.jpg

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