Hu Yuanliang, Pan Lina, Dun Yaohao, Peng Nan, Liang Yunxiang, Zhao Shumiao
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University , Wuhan 430070 , P.R. China ; College of Life Sciences, Hubei Normal University , Huangshi , Hubei 435002 , P.R. China.
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University , Wuhan 430070 , P.R. China.
Biotechnol Biotechnol Equip. 2014 Sep 3;28(5):843-849. doi: 10.1080/13102818.2014.962407. Epub 2014 Oct 22.
This study is focussed on the possibility of producing a yeast culture with yellow wine lees as a substrate by solid-state fermentation (SSF). Results showed that a yeast count of 1.58 × 10 CFU/g was achieved by signal factor and orthogonal experiments. After fermentation, the starch content in the yeast culture reduced from 32.2% ± 0.5% to 7.5% ± 0.2%, and the contents of crude protein and peptide increased from 36.1% ± 0.8% to 48.0% ± 1.0% and 3.9% ± 0.2% to 7.2% ± 0.4%, respectively. Additionally, large amounts of short peptides and free amino acids were detected by fast protein liquid chromatography (FPLC). These results suggest that yellow wine lees are a suitable substrate for the production of yeast cultures. It can serve as a growth-promoting factor and help reduce the shortage of protein feed in the animal industry. This research provides a potential way for the utilization of agro-industrial residues.
本研究聚焦于以黄酒糟为底物通过固态发酵(SSF)生产酵母培养物的可能性。结果表明,通过单因素和正交试验,酵母培养物中的酵母计数达到了1.58×10 CFU/g。发酵后,酵母培养物中的淀粉含量从32.2%±0.5%降至7.5%±0.2%,粗蛋白和肽的含量分别从36.1%±0.8%增至48.0%±1.0%以及从3.9%±0.2%增至7.2%±0.4%。此外,通过快速蛋白质液相色谱(FPLC)检测到大量的短肽和游离氨基酸。这些结果表明,黄酒糟是生产酵母培养物的合适底物。它可作为一种生长促进因子,有助于减少畜牧业中蛋白质饲料的短缺。本研究为农业工业废弃物的利用提供了一条潜在途径。