Woudwyk Mariana A, Zanuzzi Carolina N, Nishida Fabián, Gimeno Eduardo J, Soto Pedro, Monteavaro Cristina E, Barbeito Claudio G
Laboratory of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina.
Laboratory of Histology and Embryology, School of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina; Institute of Pathology, School of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina; National Scientific and Technical Council (CONICET), Argentina.
Exp Parasitol. 2015 Sep;156:32-6. doi: 10.1016/j.exppara.2015.05.013. Epub 2015 May 28.
Bovine tritrichomonosis is a sexually transmitted disease caused by the protozoon Tritrichomonas foetus and characterised by embryonic-death and abortion. During pregnancy, the processes of cell proliferation and death play a crucial role for blastocyst implantation and the subsequent maintenance of early pregnancy, and their misbalance may lead to the abortion. In this study, we aimed to investigate whether cell proliferation and death may be altered during tritrichomonosis. For this purpose, we used pregnant BALB/c mice as an alternative experimental animal model that has successfully reproduced the infection. We analysed the immunohistochemical expression of active caspase-3 and proliferating cell nuclear (PCNA) antigens in the endometrium of infected mice. We found an increase in the number of caspase-3 positive cells in infected mice that were not pregnant at the necropsy. Besides, the number of positive proliferating cells increased in the uterine luminal epithelium of infected animals killed at 5-7 days post coitum (dpc). Pregnant infected mice killed at 8-11 dpc showed higher proliferation than control animals. We suggest that the cytopathic effect induced by T. foetus in the uteri of infected mice may induce the apoptosis of the epithelial cells and, as a result, promote a compensatory proliferative response. The information described here will be helpful to further study the pathogenesis of the bovine tritrichomonosis.
牛三毛滴虫病是一种由胎儿三毛滴虫原虫引起的性传播疾病，其特征为胚胎死亡和流产。在妊娠期间，细胞增殖和死亡过程对胚泡着床及随后的早期妊娠维持起着关键作用，而它们的失衡可能导致流产。在本研究中，我们旨在调查在感染三毛滴虫病期间细胞增殖和死亡是否会发生改变。为此，我们使用怀孕的BALB/c小鼠作为一种成功再现感染的替代实验动物模型。我们分析了感染小鼠子宫内膜中活性半胱天冬酶-3和增殖细胞核抗原（PCNA）的免疫组化表达。我们发现，在尸检时未怀孕的感染小鼠中，半胱天冬酶-3阳性细胞数量增加。此外，在交配后5 - 7天（dpc）处死的感染动物的子宫腔上皮中，阳性增殖细胞数量增加。在8 - 11 dpc处死的怀孕感染小鼠显示出比对照动物更高的增殖。我们认为，胎儿三毛滴虫在感染小鼠子宫中诱导的细胞病变效应可能诱导上皮细胞凋亡，结果促进了代偿性增殖反应。此处描述的信息将有助于进一步研究牛三毛滴虫病的发病机制。
