Yan Shan, Yao Haosheng, Chen Zhen, Zeng Shengquan, Xi Xi, Wang Yuanpeng, He Ning, Li Qingbiao
Dept. of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005,, P.R. China.
Dept. of Chemistry, State University of New York at Binghamton, Binghamton, NY, 13902,, Unites States.
Biotechnol Prog. 2015 Sep-Oct;31(5):1287-94. doi: 10.1002/btpr.2118. Epub 2015 Jun 26.
As an environmentally friendly and industrially useful biopolymer, poly-γ-glutamic acid (γ-PGA) from Bacillus licheniformis CGMCC 2876 was characterized by the high-resolution mass spectrometry and (1)H NMR. A flocculating activity of 11,474.47 U mL(-1) obtained with γ-PGA, and the effects of carbon sources, ions, and chemical properties (D-/L-composition and molecular weight) on the production and flocculating activity of γ-PGA were discussed. Being a bioflocculant in the sugar refinery process, the color and turbidity of the sugarcane juice was IU 1,877.36 and IU 341.41 with 0.8 ppm of γ-PGA, respectively, which was as good as the most widely used chemically synthesized flocculant in the sugarcane industry--polyacrylamide with 1 ppm. The γ-PGA produced from B. licheniformis CGMCC 2876 could be a promising alternate of chemically synthesized flocculants in the sugarcane industry.
作为一种环境友好且具有工业用途的生物聚合物，地衣芽孢杆菌CGMCC 2876产生的聚-γ-谷氨酸（γ-PGA）通过高分辨率质谱和氢核磁共振（1H NMR）进行了表征。γ-PGA的絮凝活性为11,474.47 U mL(-1)，并讨论了碳源、离子以及化学性质（D-/L-组成和分子量）对γ-PGA产量和絮凝活性的影响。作为制糖过程中的生物絮凝剂，在添加0.8 ppm γ-PGA时，甘蔗汁的颜色和浊度分别为IU 1,877.36和IU 341.41，与甘蔗工业中使用最广泛的化学合成絮凝剂——1 ppm的聚丙烯酰胺效果相当。地衣芽孢杆菌CGMCC 2876产生的γ-PGA有望成为甘蔗工业中化学合成絮凝剂的替代物。
