Lee Alan Yueh-Luen, Fan Chi-Chen, Chen Yen-An, Cheng Chieh-Wen, Sung Ya-Ju, Hsu Chih-Ping, Kao Ting-Yu
National Institute of Cancer Research, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
Mackay Memorial Hospital Taipei, Taiwan Yuanpei University of Medical Technology, Hsinchu, Taiwan.
Integr Cancer Ther. 2015 Sep;14(5):484-90. doi: 10.1177/1534735415588930. Epub 2015 Jun 2.
Epithelial-mesenchymal transition (EMT) and invasion play a critical role in cancer progression and metastasis. We have shown that low E-cadherin and high Twist expression are significantly correlated with prognostic survival prediction in oral squamous cell carcinoma (OSCC). This study aimed to determine the anti-invasive effect of curcumin on the expression of matrix metalloproteinases (MMPs) and of EMT regulators in OSCC.
SCC-25 cells were treated with curcumin, and cell proliferation, invasion, and expression of MMPs and EMT regulators were assessed for cell viability by trypan blue exclusion, for invasion by Matrigel invasion chamber, and for EMT regulators and MMP changes in the levels of proteins by immunoblotting.
Our data showed that curcumin treatment not only decreased the expression of MMP-2 and MMP-9 to inhibit invasiveness in oral cancer but also modulated the expression of EMT markers, such as Snail, Twist, and E-cadherin, and induced p53 expression that is crucial to EMT repression.
Curcumin has the potential to become an adjunctive regimen for the prevention of cancer progression and metastasis in oral cancer.
上皮-间质转化（EMT）和侵袭在癌症进展和转移中起关键作用。我们已经表明，低E-钙黏蛋白和高Twist表达与口腔鳞状细胞癌（OSCC）的预后生存预测显著相关。本研究旨在确定姜黄素对OSCC中基质金属蛋白酶（MMPs）和EMT调节因子表达的抗侵袭作用。
用姜黄素处理SCC-25细胞，通过台盼蓝排斥法评估细胞活力，通过基质胶侵袭小室评估侵袭能力，通过免疫印迹法评估EMT调节因子和MMPs蛋白水平的变化，以此来检测细胞增殖、侵袭以及MMPs和EMT调节因子的表达。
我们的数据表明，姜黄素处理不仅降低了MMP-2和MMP-9的表达以抑制口腔癌的侵袭性，还调节了EMT标志物（如Snail、Twist和E-钙黏蛋白）的表达，并诱导了对EMT抑制至关重要的p53表达。
姜黄素有可能成为预防口腔癌进展和转移的辅助治疗方案。
