α,β-不饱和内酯对埃及伊蚊幼虫存活、肠道胰蛋白酶以及产卵反应的影响

Effects of α,β-unsaturated lactones on larval survival and gut trypsin as well as oviposition response of Aedes aegypti.

作者信息

Barros Maria Ester S B, Freitas Juliano C R, Santos Geanne K N, da Silva Rayane Cristine Santos, Pontual Emmanuel V, Paiva Patrícia M G, Napoleão Thiago H, Navarro Daniela M A F, Menezes Paulo H

机构信息

Departamento de Química Fundamental-CCEN, Universidade Federal de Pernambuco, Av. Jornalista Aníbal Fernandes, s/n - Cidade Universitária, Recife, PE 50740-560, Brazil.

Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Rua Dom Manuel de Medeiros, s/n - Dois Irmãos, Recife, PE 52171-900, Brazil.

出版信息

Exp Parasitol. 2015 Sep;156:37-41. doi: 10.1016/j.exppara.2015.05.017. Epub 2015 Jun 1.

DOI:10.1016/j.exppara.2015.05.017

PMID:26044355

Abstract

Lactones are organic cyclic esters that have been described as larvicides against Aedes aegypti and as components of oviposition pheromone of Culex quinquefasciatus. This work describes the effect of six α,β-unsaturated lactones (5a-5f) on survival of A. aegypti fourth instar larvae (L4). It is also reported the effects of the lactones on L4 gut trypsin activity and oviposition behavior of A. aegypti females. Five lactones were able to kill L4 being the lactones 5a (LC50 of 39.05 ppm), 5e (LC50 of 36.30 ppm) and 5f (LC50 of 40.46 ppm) the most promising larvicides. Only the lactone 5a inhibited L4 gut trypsin activity, with an IC50 of 115.15 µg/mL. Lactones 5a, 5c, 5d and 5e did not exert deterrent or stimulatory effects on oviposition, whereas lactone 5b exhibited a strong deterrent oviposition activity. In conclusion, this work introduces new α,β-unsaturated lactones as promising alternatives to control A. aegypti dissemination. The larvicidal mechanism of the lactone 5a can involve the disruption of proteolysis at larval gut.

摘要

内酯是有机环状酯，已被描述为针对埃及伊蚊的杀幼虫剂以及致倦库蚊产卵信息素的成分。这项工作描述了六种α,β-不饱和内酯（5a - 5f）对埃及伊蚊四龄幼虫（L4）存活的影响。还报告了这些内酯对L4肠道胰蛋白酶活性以及埃及伊蚊雌蚊产卵行为的影响。五种内酯能够杀死L4，其中内酯5a（LC50为39.05 ppm）、5e（LC50为36.30 ppm）和5f（LC50为40.46 ppm）是最有前景的杀幼虫剂。只有内酯5a抑制L4肠道胰蛋白酶活性，IC50为115.15 μg/mL。内酯5a、5c、5d和5e对产卵没有产生威慑或刺激作用，而内酯5b表现出强烈的产卵威慑活性。总之，这项工作引入了新的α,β-不饱和内酯作为控制埃及伊蚊传播的有前景的替代物。内酯5a的杀幼虫机制可能涉及破坏幼虫肠道中的蛋白质水解。