Nikkola Lila, Morton Tatjana, Balmayor Elizabeth R, Jukola Hanna, Harlin Ali, Redl Heinz, van Griensven Martijn, Ashammakhi Nureddin
Department of Biomedical Engineering, Tampere University of Technology, Tampere, Finland.
AUVA Research Center, Austrian Cluster for Tissue Regeneration, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria.
Eur J Med Res. 2015 Jun 5;20(1):54. doi: 10.1186/s40001-015-0145-1.
Adaptation of nanotechnology into materials science has also advanced tissue engineering research. Tissues are basically composed of nanoscale structures hence making nanofibrous materials closely resemble natural fibers. Adding a drug release function to such material may further advance their use in tissue repair.
In the current study, bioabsorbable poly(D,L lactide-co-glycolide)80/20 (PDLGA80/20) was dissolved in a mixture of acetone/dimethylformamide. Twenty percent of diclofenac sodium was added to the solution. Nanofibers were manufactured using electrospinning. The morphology of the obtained scaffolds was analyzed by scanning electron microscopy (SEM). The release of the diclofenac sodium was assessed by UV/Vis spectroscopy. Mouse fibroblasts (MC3T3) were seeded on the scaffolds, and the cell attachment was evaluated with fluorescent microscopy.
The thickness of electrospun nanomats was about 1 mm. SEM analysis showed that polymeric nanofibers containing drug particles formed very interconnected porous nanostructures. The average diameter of the nanofibers was 500 nm. Drug release was measured by means of UV/Vis spectroscopy. After a high start peak, the release rate decreased considerably during 11 days and lasted about 60 days. During the evaluation of the release kinetics, a material degradation process was observed. MC3T3 cells attached to the diclofenac sodium-loaded scaffold.
The nanofibrous porous structure made of PDLGA polymer loaded with diclofenac sodium is feasible to develop, and it may help to improve biomaterial properties for controlled tissue repair and regeneration.
将纳米技术应用于材料科学也推动了组织工程研究的发展。组织基本上由纳米级结构组成，因此纳米纤维材料与天然纤维非常相似。给这种材料添加药物释放功能可能会进一步推进其在组织修复中的应用。
在本研究中，将生物可吸收的聚（D，L-丙交酯-共-乙交酯）80/20（PDLGA80/20）溶解在丙酮/二甲基甲酰胺的混合物中。向该溶液中加入20%的双氯芬酸钠。使用静电纺丝制造纳米纤维。通过扫描电子显微镜（SEM）分析所得支架的形态。通过紫外/可见光谱法评估双氯芬酸钠的释放情况。将小鼠成纤维细胞（MC3T3）接种在支架上，并用荧光显微镜评估细胞附着情况。
电纺纳米垫的厚度约为1毫米。SEM分析表明，含有药物颗粒的聚合物纳米纤维形成了非常相互连接的多孔纳米结构。纳米纤维的平均直径为500纳米。通过紫外/可见光谱法测量药物释放。在一个高起始峰之后，释放速率在11天内大幅下降，并持续约60天。在评估释放动力学过程中，观察到材料降解过程。MC3T3细胞附着在负载双氯芬酸钠的支架上。
由负载双氯芬酸钠的PDLGA聚合物制成的纳米纤维多孔结构是可行的，并且可能有助于改善生物材料性能，以实现可控的组织修复和再生。
