Segovia M, Navarro A, Artero J M
Department of Microbiology, Faculty of Medicine, University of Murcia, Spain.
Ann Trop Med Parasitol. 1989 Aug;83(4):357-60. doi: 10.1080/00034983.1989.11812357.
Desferrioxamine was tested in vitro for activity against promastigotes of Leishmania donovani. In addition, the effects of liposome-encapsulated desferrioxamine and free desferrioxamine on macrophages infected with amastigotes of L. donovani were compared. The drug was added to the culture medium for three days, and the results were compared with several controls; the drug solvent, empty liposomes, and desferrioxamine which had been re-ferrated with an equimolar concentration of ferric ammonium sulphate. Desferrioxamine was found to be inactive against growing promastigotes at the highest concentration used, 50 micrograms ml-1. On the other hand, 44% and 60% of amastigotes were eliminated when macrophages infected with L. donovani were exposed to 50 micrograms ml-1 of free or liposome-encapsulated desferrioxamine respectively.
对去铁胺进行了体外抗杜氏利什曼原虫前鞭毛体活性测试。此外，还比较了脂质体包裹的去铁胺和游离去铁胺对感染杜氏利什曼原虫无鞭毛体的巨噬细胞的影响。将药物添加到培养基中持续三天，并将结果与几个对照组进行比较；药物溶剂、空脂质体以及用等摩尔浓度硫酸铁铵重新铁化的去铁胺。发现在所用最高浓度50微克/毫升时，去铁胺对生长中的前鞭毛体无活性。另一方面，当感染杜氏利什曼原虫的巨噬细胞分别暴露于50微克/毫升的游离或脂质体包裹的去铁胺时，分别有44%和60%的无鞭毛体被清除。
