Zhang Tiancheng, Li Jing, Xia Tian, Zhang Nan, Zhang Yaozhou, Zhao Jian
International Joint Cancer Institute, The Second Military Medical University Shanghai, China.
Tianjin East Innovation Biotechnology Limited Company Tianjin, China.
Int J Clin Exp Pathol. 2015 Mar 1;8(3):3168-73. eCollection 2015.
To explore the association between COX-2 polymorphisms and non-small cell lung cancer (NSCLC) susceptibility.
We collected fasting peripheral venous blood from 60 cases with NSCLC and 62 healthy controls through physical examinations, and applied PCR-RFLP to analyze COX-2 polymorphisms of two groups.
With respect to detecting COX-2 rs689466 and rs5275 polymorphisms, the distribution frequency of mutant genotype AA of COX-2 rs689466 in case group was higher than that in control group, which possessed significant difference between two groups (P<0.05). Carriers with AA genotype were 4.05 times at risk of NSCLC than those with GG genotype (P = 0.04, OR=4.05, 95% CI=1.14-14.43). The distribution of mutant genotype CC of COX-2 rs5275 was different between two groups, and carriers with genotype CC were at 5.70 times higher risk of NSCLC than those with genotype TT. After corrected by sex, gender, smoking and drinking factors, AA genotype of COX-2 rs689466 and CC genotype of COX-2 rs5275 still contributed to increased risk of NSCLC (OR=4.22, 95% CI=1.10-16.17, OR=6.95, 95% CI=1.27-38.11). After analyzed of linkage disequilibrium (LD) and haplotypes of alleles in two SNPs, the distribution frequency of A-C haplotype in case group was higher than that in control group, with significant difference between two groups (P<0.05). After corrected by sex, gender, smoking and drinking factors, statistical difference was still found in the total distribution of A-C haplotype between two groups (P=0.03, OR=6.11, 95% CI=1.16-32.2).
COX-2 rs689466 and rs5275 polymorphisms may be related to NSCLC susceptibility. And A-C haplotype might be a susceptibility haplotype for NSCLC.
探讨COX - 2基因多态性与非小细胞肺癌（NSCLC）易感性之间的关联。
通过体检收集60例NSCLC患者和62例健康对照者的空腹外周静脉血，应用聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）分析两组的COX - 2基因多态性。
在检测COX - 2 rs689466和rs5275多态性方面，病例组中COX - 2 rs689466突变基因型AA的分布频率高于对照组，两组间差异有统计学意义（P<0.05）。AA基因型携带者患NSCLC的风险是GG基因型携带者的4.05倍（P = 0.04，OR = 4.05，95%CI = 1.14 - 14.43）。COX - 2 rs5275突变基因型CC在两组间的分布不同，CC基因型携带者患NSCLC的风险比TT基因型携带者高5.70倍。经性别、吸烟和饮酒因素校正后，COX - 2 rs689466的AA基因型和COX - 2 rs5275的CC基因型仍与NSCLC风险增加有关（OR = 4.22，95%CI = 1.10 - 16.17，OR = 6.95，95%CI = 1.27 - 38.11）。对两个单核苷酸多态性（SNP）的等位基因进行连锁不平衡（LD）和单倍型分析后，病例组中A - C单倍型的分布频率高于对照组，两组间差异有统计学意义（P<0.05）。经性别、吸烟和饮酒因素校正后，两组间A - C单倍型的总体分布仍存在统计学差异（P = 0.03，OR = 6.11，95%CI = 1.16 - 32.2）。
COX - 2 rs689466和rs5275多态性可能与NSCLC易感性有关。且A - C单倍型可能是NSCLC的易感单倍型。
