Lin Zi Li, Cui Xiang-Shun, Namgoong Suk, Kim Nam-Hyung
Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea.
J Reprod Dev. 2015;61(5):361-7. doi: 10.1262/jrd.2015-021. Epub 2015 Jun 6.
Junction-mediating and regulatory protein (JMY) is a regulator of both transcription and actin filament assembly. JMY is a critical nucleation-promoting factor (NPF); however, its role in the development of mammalian embryos is poorly understood. In the current study, we investigated the functional roles of the NPF JMY in porcine embryos. Porcine embryos expressed JMY mRNA and protein, and JMY protein moved from the cytoplasm to the nucleus at later embryonic developmental stages. Knockdown of JMY by RNA interference markedly decreased the rate of blastocyst development, validating its role in the development of porcine embryos. Furthermore, injection of JMY dsRNA also impaired actin and Arp2 expression, and co-injection of actin and Arp2 mRNA partially rescued blastocyst development. Taken together, our results show that the NPF JMY is involved in the development of porcine embryos by regulating the NPF-Arp2-actin pathway.
连接介导与调节蛋白（JMY）是转录和肌动蛋白丝组装的调节因子。JMY是一种关键的成核促进因子（NPF）；然而，其在哺乳动物胚胎发育中的作用尚不清楚。在本研究中，我们研究了NPF JMY在猪胚胎中的功能作用。猪胚胎表达JMY mRNA和蛋白，并且JMY蛋白在胚胎发育后期从细胞质转移到细胞核。通过RNA干扰敲低JMY显著降低了囊胚发育率，证实了其在猪胚胎发育中的作用。此外，注射JMY双链RNA也损害了肌动蛋白和Arp2的表达，而共注射肌动蛋白和Arp2 mRNA部分挽救了囊胚发育。综上所述，我们的结果表明，NPF JMY通过调节NPF-Arp2-肌动蛋白途径参与猪胚胎的发育。
