Liu Peng, Zhao Wen-Ge
a College of Life Science and Technology, Harbin Normal University , Harbin , Heilongjiang , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2742-3. doi: 10.3109/19401736.2015.1046174. Epub 2015 Jun 9.
In this study, the complete mitogenome sequence of Elaphe anomala (Squamata: Colubridae) is first determined using long PCR. It is a circular molecule of 17,164 bp in length and contains 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes and 2 control regions (CRI and CRII). The gene order and nucleotide composition of E. anomala are very similar with E. schrenckii. Mitochondrial genomes analyses based on the NJ method yield phylogenetic tree of 17 species snakes of Colubridae. Species E. anomala, E. schrenckii, E. bimaculata and E. davidi seemed to have formed a monophyletic group with the high bootstrap value (100%) except E. poryphyracea. Oligodon ningshaanensis and Thermophis zhaoermii are special species. The molecular data presented here provide a useful tool for setting the stage for further studies.
在本研究中，首次使用长PCR技术测定了赤峰锦蛇（有鳞目：游蛇科）的完整线粒体基因组序列。它是一个长度为17,164 bp的环状分子，包含13个蛋白质编码基因（PCGs）、22个转运RNA（tRNA）基因、2个核糖体RNA（rRNA）基因和2个控制区（CRI和CRII）。赤峰锦蛇的基因顺序和核苷酸组成与棕黑锦蛇非常相似。基于NJ法的线粒体基因组分析产生了游蛇科17种蛇的系统发育树。除紫灰锦蛇外，赤峰锦蛇、棕黑锦蛇、双斑锦蛇和王锦蛇似乎形成了一个具有高自展值（100%）的单系类群。宁陕小头蛇和赵氏喜山蛇是特殊物种。这里呈现的分子数据为进一步研究奠定了基础，提供了一个有用的工具。
