Banche Giuliana, Prato Mauro, Magnetto Chiara, Allizond Valeria, Giribaldi Giuliana, Argenziano Monica, Khadjavi Amina, Gulino Giulia Rossana, Finesso Nicole, Mandras Narcisa, Tullio Vivian, Cavalli Roberta, Guiot Caterina, Cuffini Anna Maria
Dipartimento di Scienze della Sanità Pubblica e Pediatriche, Università di Torino, Torino, Italy.
Dipartimento di Neuroscienze, Università di Torino, Torino, Italy.
Future Microbiol. 2015;10(6):929-39. doi: 10.2217/fmb.15.27.
Chronic wounds, characterized by hypoxia, inflammation and impaired tissue remodeling, are often worsened by bacterial/fungal infections. Intriguingly, chitosan-shelled/decafluoropentane-cored oxygen-loaded nanodroplets (OLNs) have proven effective in delivering oxygen to hypoxic tissues.
The present work aimed at investigating nanodroplet antimicrobial properties against methicillin-resistant Staphylococcus aureus (MRSA) or Candida albicans, toxicity on human keratinocytes (HaCaT) and ultrasound (US)-triggered transdermal delivery.
MATERIALS & METHODS: Nanodroplet antibacterial/antifungal properties, human cytotoxicity, and US-triggered transdermal delivery were measured through microbiological, biochemical, and sonophoresis assays, respectively.
OLNs and oxygen-free nanodroplets (OFNs) displayed short- or long-term cytostatic activity against MRSA or Candida albicans, respectively. OLNs were not toxic to keratinocytes, whereas OFNs slightly affected cell viability. Complementary US treatment promoted OLN transdermal delivery.
As such, US-activated chitosan-shelled OLNs appear as promising, nonconventional and innovative tools for adjuvant treatment of infected chronic wounds.
慢性伤口的特征是缺氧、炎症和组织重塑受损，常因细菌/真菌感染而恶化。有趣的是，壳聚糖包裹/十氟戊烷为核心的载氧纳米液滴（OLNs）已被证明能有效地向缺氧组织输送氧气。
本研究旨在探究纳米液滴对耐甲氧西林金黄色葡萄球菌（MRSA）或白色念珠菌的抗菌特性、对人角质形成细胞（HaCaT）的毒性以及超声（US）触发的经皮递送。
分别通过微生物学、生物化学和超声透皮给药试验来测定纳米液滴的抗菌/抗真菌特性、人体细胞毒性和超声触发的经皮递送。
OLNs和无氧纳米液滴（OFNs）分别对MRSA或白色念珠菌显示出短期或长期的抑菌活性。OLNs对角质形成细胞无毒，而OFNs对细胞活力有轻微影响。辅助超声治疗可促进OLN的经皮递送。
因此，超声激活的壳聚糖包裹OLNs似乎是用于辅助治疗感染性慢性伤口的有前景、非常规且创新的工具。
