malS的5'-非翻译区通过影响bax的表达增加伤寒沙门氏菌的侵袭能力。
5'-UTR of malS increases the invasive capacity of Salmonella enterica serovar Typhi by influencing the expression of bax.
作者信息
Gong Mingyu, Xu Shungao, Jin Yue, Zhang Ying, Dadzie Isaac, Zhang Xiaolei, Wang Zhexin, Zhu Yunxia, Ni Bin, Sheng Xiumei, Huang Xinxiang
机构信息
Department of Biochemistry & Molecular Biology, School of Medical Science & Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Department of Biochemistry, Chengde Medical College, Chengde, Hebei 067000, China.
出版信息
Future Microbiol. 2015;10(6):941-54. doi: 10.2217/fmb.15.12.
AIM
An RNA-seq analysis recently identified a 236-nucleotide transcript upstream from malS in Salmonella enterica serovar Typhi. Here, we investigated its molecular characteristics and function.
MATERIALS & METHODS: RACE and northern blotting were used to determine the molecular characteristics of the sequence, and mutagenesis, microarray, immunoblotting and an invasion assay were used to investigate the functions of the transcript.
RESULTS
The transcript was identified as the malS 5'-untranslated region (UTR), which could influence the expression of the flagellar and SPI-1 genes and the invasion of HeLa cells by S. Typhi. Deletion of bax increased the expression of the invasion genes and the invasive capacity of S. Typhi, whereas the expression of the malS 5'-UTR reduced the expression of bax.
CONCLUSION
The malS 5'-UTR reduces the expression of bax and increases the invasive capacity of S. Typhi.
目的
最近一项RNA测序分析在伤寒沙门氏菌中鉴定出位于malS上游的一段236个核苷酸的转录本。在此，我们研究了其分子特征和功能。
材料与方法
采用RACE和Northern印迹法确定该序列的分子特征，并用诱变、微阵列、免疫印迹和侵袭试验研究该转录本的功能。
结果
该转录本被鉴定为malS 5'-非翻译区（UTR），它可影响鞭毛和SPI-1基因的表达以及伤寒沙门氏菌对HeLa细胞的侵袭。缺失bax可增加侵袭基因的表达和伤寒沙门氏菌的侵袭能力，而malS 5'-UTR的表达则降低了bax的表达。
结论
malS 5'-UTR降低了bax的表达并增加了伤寒沙门氏菌的侵袭能力。
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