Keepers Kyle, Martin Andrew P, Kane Nolan C
a Department of Ecology and Evolutionary Biology , University of Colorado , Boulder , CO , USA.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2349-50. doi: 10.3109/19401736.2015.1025259. Epub 2015 Jun 10.
In this article we report the complete mitochondrial genome of the Warm Springs pupfish, Cyprinodon nevadensis pectoralis. The genomic DNA of a single female individual was extracted and sequenced on the Illumina HiSeq2000 platform. It contains 16,499 bp and a total of 37 genes, divided into 22 tRNA genes, 2 rRNA genes and 13 protein-coding genes. It exhibits 94% sequence similarity with the other published mitochondrion in its genus, C. rubrofluviatilis. A Tamura-Nei maximum-likelihood tree constructed from mitochondrial sequences shows expected phylogenetic relationships between C. nevadensis and sister taxa.
在本文中，我们报告了温泉鳉鱼（Cyprinodon nevadensis pectoralis）的完整线粒体基因组。从单个雌性个体中提取基因组DNA，并在Illumina HiSeq2000平台上进行测序。它包含16,499个碱基对，共有37个基因，分为22个tRNA基因、2个rRNA基因和13个蛋白质编码基因。它与该属中已发表的另一种线粒体——红溪鳉（C. rubrofluviatilis）的序列相似度为94%。根据线粒体序列构建的 Tamura-Nei 最大似然树显示了内华达鳉与姐妹分类群之间预期的系统发育关系。
