Zhao Jian, Li Wei, Zhang Dandan, Wen Ping, Zhu Xinping
a Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture , Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences , Guangzhou , P.R. China and.
b College of Fisheries and Life Science, Shanghai Ocean University , Shanghai , P.R . China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2466-7. doi: 10.3109/19401736.2015.1033700. Epub 2015 Jun 10.
The complete mitochondrial genomes of three lineages (N, TW and S) of Mauremys mutica are determined in this study. The total lengths of the mitogenomes were 16,758 bp for N, 16 500bp for TW, and 16 494bp for S. The nucleotide composition was 26.3-27% for T, 26.2-26.8% for C, and 33.8-33.9% for A. The genomes encoded 37 genes typically found in other vertebrates. Three CSBs were identified, and the CSB1 were variable. A long tandem repeats of (TTATTATA) 30 were found in the control region of N mitogenome, but none in TW and S lineage. These sequences would be useful for the phylogenetic and conservation studies of Asian endangered turtles.
本研究测定了黄喉拟水龟三个谱系(N、TW和S)的完整线粒体基因组。N谱系线粒体基因组全长16758bp,TW谱系为16500bp,S谱系为16494bp。核苷酸组成中,T占26.3 - 27%,C占26.2 - 26.8%,A占33.8 - 33.9%。这些基因组编码了其他脊椎动物中常见的37个基因。鉴定出三个保守序列块(CSB),且CSB1存在变异。在N谱系线粒体基因组的控制区发现了一段由30个(TTATTATA)组成的长串联重复序列,但在TW和S谱系中未发现。这些序列将有助于亚洲濒危龟类的系统发育和保护研究。