Yang Hong, Wu Yumei, Li Haoxue, Gao Yongming, Qu Lin, Yang Jingyuan, Tao Bo
Department of AIDS/STD Control and Prevention, Inner Mongolia Center for Disease Control and Prevention Hohhot 010031, Inner Mongolia, China.
Int J Clin Exp Med. 2015 Mar 15;8(3):4250-6. eCollection 2015.
Estimation of T-lymphocyte subsets continues to be an important aspect for monitoring HIV disease progression and response to antiretroviral therapy. Most of the diagnostic laboratories often rely on studies from western for CD4+T-lymphocyte reference values, which could, often be unreliable for usage in local settings. To establish the normal reference values of T lymphocyte subsets from healthy people of Inner Mongolia Autonomous Region, flow cytometry was performed to determine the reference ranges for lymphocyte subsets (CD3 and CD4 cells) in 400 healthy multiracial adult population from 12 League Cities in Inner Mongolia Region, China. The basic information including age, gender, nationality and history was collected. There were significant differences in the absolute counting, percentage of CD3+T lymphocytes, and CD4+T lymphocyte percentage counting among different age groups. There were significant differences in CD3+, CD4+T lymphocyte percentage in the groups with different genders. There were significant differences in CD3+T lymphocyte percentage count, absolute count of CD4+T lymphocytes and CD4+T lymphocyte percentage counting in the group with ages of 16-20. There were dramatic differences in CD3+T lymphocyte percentage count and CD4+T lymphocyte percentage counting in the group with ages of 31-40. There were significant differences in CD4+T lymphocyte percentage counting. By this study, age, gender and ethnic specific lymphocyte subset reference ranges have been locally established in Inner Mongolia Autonomous Region.
T淋巴细胞亚群的评估仍然是监测HIV疾病进展和对抗逆转录病毒治疗反应的一个重要方面。大多数诊断实验室常常依赖西方的研究来获取CD4+T淋巴细胞的参考值，而这些参考值在当地环境中使用时往往不可靠。为了确定内蒙古自治区健康人群T淋巴细胞亚群的正常参考值，采用流式细胞术对来自中国内蒙古地区12个盟市的400名健康多民族成年人群的淋巴细胞亚群（CD3和CD4细胞）参考范围进行了测定。收集了包括年龄、性别、民族和病史等基本信息。不同年龄组之间CD3+T淋巴细胞的绝对计数、百分比以及CD4+T淋巴细胞百分比计数存在显著差异。不同性别组之间CD3+、CD4+T淋巴细胞百分比存在显著差异。16 - 20岁组的CD3+T淋巴细胞百分比计数、CD4+T淋巴细胞绝对计数和CD4+T淋巴细胞百分比计数存在显著差异。31 - 40岁组的CD3+T淋巴细胞百分比计数和CD4+T淋巴细胞百分比计数存在显著差异。CD4+T淋巴细胞百分比计数存在显著差异。通过本研究，在内蒙古自治区已在当地建立了年龄、性别和民族特异性的淋巴细胞亚群参考范围。
