Wood P J, Socarras S, Streilein J W
Department of Microbiology and Immunology, University of Miami School of Medicine, Florida.
Immunology. 1989 Dec;68(4):503-6.
The specificity of the residual anti-B10 cytotoxic T-cell response of B10. A mice rendered neonatally tolerant of B10 was compared to the anti-B10 response of adult and neonatal normal B10. A mice. The response of both spleen cells and thymus cells from adult and neonatal normal mice was biased toward Kb. This was in contrast to the response from tolerant spleen which was biased toward Db. The results suggest that the repertoire of normal mice is established neonatally and does not change radically without specific antigenic challenge. Furthermore the fact that the residual tolerogen specific cytotoxic T-cell precursors (pTc) in tolerant mice have a different repertoire to normal neonates makes it unlikely that they are remnants of a neonatal repertoire that developed prior to the full establishment of tolerance. Taking into account the present and previous results, the residual tolerogen-specific Tc in tolerant mice most likely represent a population of cells that has escaped tolerance induction due to their low avidity for antigen and provide the first evidence that avidity plays a role in tolerance among cytotoxic T cells.
将新生期耐受B10的B10.A小鼠残余抗B10细胞毒性T细胞反应的特异性，与成年和新生期正常B10.A小鼠的抗B10反应进行了比较。成年和新生期正常小鼠的脾细胞和胸腺细胞反应均偏向于Kb。这与耐受脾细胞偏向于Db的反应形成对比。结果表明，正常小鼠的细胞库在新生期就已建立，并且在没有特异性抗原刺激的情况下不会发生根本性变化。此外，耐受小鼠中残余的耐受原特异性细胞毒性T细胞前体（pTc）与正常新生小鼠具有不同的细胞库，这使得它们不太可能是在耐受完全建立之前发育的新生细胞库的残余物。综合目前和先前的结果，耐受小鼠中残余的耐受原特异性Tc很可能代表了一群由于对抗原亲和力低而逃避耐受诱导的细胞，并首次提供了亲和力在细胞毒性T细胞耐受中起作用的证据。
