Wood P J, Streilein J W
Eur J Immunol. 1982 Mar;12(3):188-94. doi: 10.1002/eji.1830120304.
The ontogeny of mixed lymphocyte reaction and cell-mediated lymphocytotoxicity (CML) responsiveness was studied in mice that had been neonatally tolerized by the injection of 15 X 10(6) semiallogeneic bone marrow and spleen cells, and compared to that of unprimed littermates. Spleen cells from primed mice did not develop reactivity towards the tolerizing antigen but did develop reactivity towards third-party alloantigens. Similarily, thymus cells from primed mice also failed to develop CML reactivity towards the tolerizing antigen. Thymus cell mixed lymphocyte reactivity, which was demonstrable in neonates before the tolerizing injection, declined rapidly between 2 and 4 days of age, compared to that of controls. Thus neonatal tolerance was shown to operate at least at a thymic, and perhaps pre-thymic level; measurement of the levels of chimeric cells in the thymus and bone marrow favored the latter interpretation.
在新生期通过注射15×10⁶半同种异体骨髓和脾细胞而产生耐受的小鼠中，研究了混合淋巴细胞反应和细胞介导的淋巴细胞毒性（CML）反应性的个体发生，并与未致敏的同窝小鼠进行了比较。致敏小鼠的脾细胞对耐受抗原未产生反应性，但对第三方同种异体抗原有反应性。同样，致敏小鼠的胸腺细胞对耐受抗原也未产生CML反应性。与对照组相比，在耐受注射前的新生儿中可证明的胸腺细胞混合淋巴细胞反应性在2至4日龄之间迅速下降。因此，新生期耐受至少在胸腺水平，也许在胸腺前水平起作用；对胸腺和骨髓中嵌合细胞水平的测量支持后一种解释。
