Simultaneous production of antibodies against T3 and T4 in a single animal; their characteristics and usefulness in radioimmunoassay.

Simultaneous production of antibodies against triiodothyronine and thyroxine was attempted in a single rabbit by immunizing the animal with T3-albumin conjugate and T4-albumin conjugate at the same time. This resulted in the production of antibodies against both T3 and T4. The characteristics of each antibody were studied and the antiserum was used for determination of serum T3 and T4 concentrations by radioimmunoassay. The results were compared with those obtained with antibodies against T3 and T4 which were produced individually in different rabbits and were found to be more or less similar. From the results of the experiments, it appears that it is possible to make antibodies against T3 and T4 simultaneously in a single rabbit.